| Literature DB >> 25117223 |
Abstract
The hemagglutination assay is a simple and easy method to obtain semi-quantitative data on the sugar binding and specificity of a lectin. An active lectin agglutinates erythrocytes by recognizing a carbohydrate on the cell surface and forming a cross-linked network in suspension. By serially diluting the lectin in a 96-well microtiter plate and adding a constant quantity of erythrocytes, the lectin activity can be estimated.Entities:
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Year: 2014 PMID: 25117223 DOI: 10.1007/978-1-4939-1292-6_4
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745