Evangelia Zvintzou1, George Skroubis2, Angelika Chroni3, Peristera-Ioanna Petropoulou1, Christina Gkolfinopoulou3, George Sakellaropoulos4, Donald Gantz5, Ioanna Mihou1, Fotios Kalfarentzos2, Kyriakos E Kypreos6. 1. Department of Pharmacology, University of Patras, School of Medicine, Rio Achaias, Greece. 2. Morbid Obesity Unit, Department of Surgery, University of Patras, School of Medicine, Rio Achaias, Greece. 3. Institute of Biosciences and Applications, National Center for Scientific Research Demokritos, Agia Paraskevi, Athens, Greece. 4. Department of Medical Physics, University of Patras, School of Medicine, Rio Achaias, Greece. 5. Department of Physiology and Biophysics, Boston University School of Medicine, Boston, MA, USA. 6. Department of Pharmacology, University of Patras, School of Medicine, Rio Achaias, Greece. Electronic address: kkypreos@med.upatras.gr.
Abstract
BACKGROUND: In addition to high-density lipoprotein cholesterol (HDL-C) levels, HDL quality appears also very important for atheroprotection. Obese patients with metabolic syndrome have significantly reduced HDL-C levels and are usually at increased risk for coronary heart disease. Despite that weight loss benefits these patients, its effects on HDL quality and functionality is currently poorly studied. OBJECTIVES: We investigated how rapid weight loss affects HDL structure and its antioxidant potential in patients undergoing a malabsorptive bariatric procedure. METHODS: Fasting plasma samples were collected the day before and 6 months after the bariatric procedure from 20 morbidly obese patients with body mass index >50, then HDL was isolated and analyzed by biochemical techniques. RESULTS: We report a dramatic alteration in the apolipoprotein ratio of HDL that was accompanied by the presence of more mature HDL subspecies and a concomitant increase in the antioxidant potential of HDL. Interestingly, our obese cohort could be distinguished into 2 subgroups. In 35% of patients (n = 7), HDL before surgery had barely detectable apolipoprotein (apo) A-I and apoCIII, and the vast majority of their HDL cholesterol was packed in apoE-containing HDL particles. In the remaining 65% of patients (n = 13), HDL before surgery contained high levels of apoA-I and apoCIII, in addition to apoE. In both subgroups, surgical weight loss resulted in a switch from apoE to apoA-I-containing HDL. CONCLUSIONS: Rapid weight loss exerts a significant improvement in HDL structure and functionality that may contribute to the documented beneficial effect of malabsorptive bariatric procedures on cardiovascular health.
BACKGROUND: In addition to high-density lipoprotein cholesterol (HDL-C) levels, HDL quality appears also very important for atheroprotection. Obesepatients with metabolic syndrome have significantly reduced HDL-C levels and are usually at increased risk for coronary heart disease. Despite that weight loss benefits these patients, its effects on HDL quality and functionality is currently poorly studied. OBJECTIVES: We investigated how rapid weight loss affects HDL structure and its antioxidant potential in patients undergoing a malabsorptive bariatric procedure. METHODS: Fasting plasma samples were collected the day before and 6 months after the bariatric procedure from 20 morbidly obesepatients with body mass index >50, then HDL was isolated and analyzed by biochemical techniques. RESULTS: We report a dramatic alteration in the apolipoprotein ratio of HDL that was accompanied by the presence of more mature HDL subspecies and a concomitant increase in the antioxidant potential of HDL. Interestingly, our obese cohort could be distinguished into 2 subgroups. In 35% of patients (n = 7), HDL before surgery had barely detectable apolipoprotein (apo) A-I and apoCIII, and the vast majority of their HDL cholesterol was packed in apoE-containing HDL particles. In the remaining 65% of patients (n = 13), HDL before surgery contained high levels of apoA-I and apoCIII, in addition to apoE. In both subgroups, surgical weight loss resulted in a switch from apoE to apoA-I-containing HDL. CONCLUSIONS: Rapid weight loss exerts a significant improvement in HDL structure and functionality that may contribute to the documented beneficial effect of malabsorptive bariatric procedures on cardiovascular health.
Authors: J Gómez-Ambrosi; P Andrada; V Valentí; F Rotellar; C Silva; V Catalán; A Rodríguez; B Ramírez; R Moncada; J Escalada; J Salvador; G Frühbeck Journal: Int J Obes (Lond) Date: 2017-06-06 Impact factor: 5.095
Authors: Constantine E Kosmas; Ian Martinez; Andreas Sourlas; Kyriaki V Bouza; Frederick N Campos; Verenisse Torres; Peter D Montan; Eliscer Guzman Journal: Drugs Context Date: 2018-03-28
Authors: W S Davidson; T H Inge; H Sexmith; A Heink; D Elder; D Y Hui; J T Melchior; T Kelesidis; A S Shah Journal: Int J Obes (Lond) Date: 2016-10-26 Impact factor: 5.551
Authors: Krzysztof Prusik; Jakub Kortas; Katarzyna Prusik; Jan Mieszkowski; Joanna Jaworska; Wojciech Skrobot; Marcin Lipinski; Ewa Ziemann; Jedrzej Antosiewicz Journal: Front Endocrinol (Lausanne) Date: 2018-02-20 Impact factor: 5.555
Authors: Kyriakos E Kypreos; Eleni A Karavia; Caterina Constantinou; Aikaterini Hatziri; Christina Kalogeropoulou; Eva Xepapadaki; Evangelia Zvintzou Journal: J Biomed Res Date: 2017-11-01