INTRODUCTION: Resistant bacteria are a well-known public health problem. This study was conducted to investigate the prevalence and genetic characteristics of extended spectrum β-lactamase (ESBL) producing enterobacteria, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) in mixed minced meat from pork and beef. METHODS: One hundred samples of mixed minced meat were collected from supermarkets (n = 70) and local butcher shops (n = 30) in the city of Graz (Austria). After enrichment and inoculation on selective media, bacteria were identified with MALDI-TOF MS or Vitek2 systems, tested for antibiotic resistance and further characterized with PCR and sequencing. RESULTS: In 20 of the 100 meat samples 24 ESBL positive Escherichia coli isolates were found. The most common ESBL among the isolates was CTX-M-1. Other detected bla genes contained CTX-M-14, CTX-M-32, SHV-12 and TEM-52 types. Nine samples were tested positive for MRSA and spa-typed. Detected spa-types were hospital-acquired t3928, as well as livestock-associated t011, t034 and t2241. No VRE were found. CONCLUSION: A contamination of meat with ESBL-producing E. coli and MRSA was confirmed in this study. The large diversity of ESBL producing E. coli could indicate a growing dissemination of ESBL genes in E. coli found in meat products from porcine and bovine origin.
INTRODUCTION: Resistant bacteria are a well-known public health problem. This study was conducted to investigate the prevalence and genetic characteristics of extended spectrum β-lactamase (ESBL) producing enterobacteria, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE) in mixed minced meat from pork and beef. METHODS: One hundred samples of mixed minced meat were collected from supermarkets (n = 70) and local butcher shops (n = 30) in the city of Graz (Austria). After enrichment and inoculation on selective media, bacteria were identified with MALDI-TOF MS or Vitek2 systems, tested for antibiotic resistance and further characterized with PCR and sequencing. RESULTS: In 20 of the 100 meat samples 24 ESBL positive Escherichia coli isolates were found. The most common ESBL among the isolates was CTX-M-1. Other detected bla genes contained CTX-M-14, CTX-M-32, SHV-12 and TEM-52 types. Nine samples were tested positive for MRSA and spa-typed. Detected spa-types were hospital-acquired t3928, as well as livestock-associated t011, t034 and t2241. No VRE were found. CONCLUSION: A contamination of meat with ESBL-producing E. coli and MRSA was confirmed in this study. The large diversity of ESBL producing E. coli could indicate a growing dissemination of ESBL genes in E. coli found in meat products from porcine and bovine origin.
Authors: Herbert Galler; Josefa Luxner; Christian Petternel; Franz F Reinthaler; Juliana Habib; Doris Haas; Clemens Kittinger; Peter Pless; Gebhard Feierl; Gernot Zarfel Journal: Antibiotics (Basel) Date: 2021-04-20
Authors: Cliodna A M McNulty; Donna M Lecky; Li Xu-McCrae; Deborah Nakiboneka-Ssenabulya; Keun-Taik Chung; Tom Nichols; Helen Lucy Thomas; Mike Thomas; Adela Alvarez-Buylla; Kim Turner; Sahida Shabir; Susan Manzoor; Stephen Smith; Linda Crocker; Peter M Hawkey Journal: J Antimicrob Chemother Date: 2018-05-01 Impact factor: 5.790
Authors: Gernot Zarfel; Herbert Galler; Josefa Luxner; Christian Petternel; Franz F Reinthaler; Doris Haas; Clemens Kittinger; Andrea J Grisold; Peter Pless; Gebhard Feierl Journal: Int J Environ Res Public Health Date: 2014-12-04 Impact factor: 3.390
Authors: Ethan R Wyrsch; Piklu Roy Chowdhury; Toni A Chapman; Ian G Charles; Jeffrey M Hammond; Steven P Djordjevic Journal: Front Microbiol Date: 2016-06-15 Impact factor: 5.640