Vlasta Peržeľová1, Lenka Varinská2, Barbora Dvořánková3, Pavol Szabo3, Peter Spurný4, Jaroslav Valach5, Ján Mojžiš2, Sabine André6, Hans-Joachim Gabius6, Karel Smetana7, Peter Gál8. 1. Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Department of Pathological Anatomy, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic. 2. Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic. 3. Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic. 4. Department of Cardiology, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic. 5. Department of Stomatology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic. 6. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilian University, Munich, Germany. 7. Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz. 8. Department of Pharmacology, Pavol Jozef Šafárik University, Košice, Slovak Republic Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic Department of Biomedical Research, East-Slovak Institute of Cardiovascular Diseases Inc., Košice, Slovak Republic galovci@yahoo.com pgal@vusch.sk karel.smetana@lf1.cuni.cz.
Abstract
BACKGROUND/AIM: Stromal cells in the tumor microenvironment are primarily considered as sources of promalignant factors. The objective of our study was to define the effect of extracellular matrix (ECM) produced by normal dermal or cancer-associated fibroblasts exposed to adhesion/growth-regulatory lectin galectin-1 on human umbilical vein endothelial cells (HUVECs). MATERIALS AND METHODS: Fibroblasts were cultured for 10 days with lectin, followed by removing cellular constituents after an osmotic shock. Freshly-isolated HUVECs were placed on the ECM. In parallel, HUVECs were seeded on untreated and gelatin-coated surfaces as controls. A positive control for growth of HUVECs culture using medium supplemented with vascular endothelial growth factor completed the test panel. Cells were kept in contact to the substratum for two days and then processed for immunocytochemistry. RESULTS: HUVECs seeded on fibroblast-generated ECM presented a comparatively high degree of proliferation. Furthermore, contact to substratum produced by tumor-associated fibroblasts led to generation of a meshwork especially rich in fibronectin. CONCLUSION: Galectin-1 is apparently capable to trigger ECM production favorable for growth of HUVECs, prompting further work on characterizing structural features of the ECM and in situ correlation of lectin presence, ECM constitution and neoangiogenesis. Copyright
BACKGROUND/AIM: Stromal cells in the tumor microenvironment are primarily considered as sources of promalignant factors. The objective of our study was to define the effect of extracellular matrix (ECM) produced by normal dermal or cancer-associated fibroblasts exposed to adhesion/growth-regulatory lectin galectin-1 on human umbilical vein endothelial cells (HUVECs). MATERIALS AND METHODS: Fibroblasts were cultured for 10 days with lectin, followed by removing cellular constituents after an osmotic shock. Freshly-isolated HUVECs were placed on the ECM. In parallel, HUVECs were seeded on untreated and gelatin-coated surfaces as controls. A positive control for growth of HUVECs culture using medium supplemented with vascular endothelial growth factor completed the test panel. Cells were kept in contact to the substratum for two days and then processed for immunocytochemistry. RESULTS: HUVECs seeded on fibroblast-generated ECM presented a comparatively high degree of proliferation. Furthermore, contact to substratum produced by tumor-associated fibroblasts led to generation of a meshwork especially rich in fibronectin. CONCLUSION:Galectin-1 is apparently capable to trigger ECM production favorable for growth of HUVECs, prompting further work on characterizing structural features of the ECM and in situ correlation of lectin presence, ECM constitution and neoangiogenesis. Copyright
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