Bin-Na Lee1, Kkot-Nim Lee2, Jeong-Tae Koh3, Kyung-San Min4, Hoon-Sang Chang5, In-Nam Hwang5, Yun-Chan Hwang6, Won-Mann Oh7. 1. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea; Research Center for Biomineralization Disorders, Chonnam National University, Gwangu, Korea. 2. Department of Pharmacology and Dental Therapeutics, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. 3. Research Center for Biomineralization Disorders, Chonnam National University, Gwangu, Korea; Department of Pharmacology and Dental Therapeutics, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. 4. Department of Conservative Dentistry, School of Dentistry, Chonbuk National University, Jeonju, Korea. 5. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. 6. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea; Research Center for Biomineralization Disorders, Chonnam National University, Gwangu, Korea. Electronic address: ychwang@chonnam.ac.kr. 7. Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, Korea. Electronic address: wmoh@chonnam.ac.kr.
Abstract
INTRODUCTION: Because a root-end filling material comes into contact with the surrounding cells or tissues, understanding the cell-material interfacial activity is important. Thus, the purpose of this study was to assess the biocompatibility of 3 endodontic bioactive cements (MTA [Dentsply, Tulsa, OK], Bioaggregate [BA; Innovative Bioceramix, Vancouver, BC, Canada], and Biodentine [BD; Septodont, St Maur des Fosses, France]) and to investigate the effect of cements on the differentiation of mesenchymal stem cells. METHODS: Cell viability, mineralization, and differentiation were evaluated using an 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay and alkaline phosphatase (ALP) staining. The expressions of ALP, osteocalcin, and bone sialoprotein at the gene level were detected by reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. RESULTS: Cell viability of BD in concentrations of 1, 1/2, and 1/4 was significantly lower than MTA and BA (P < .05). There was no statistically significant difference in cell viability between materials in concentrations of 1/10 and 1/50 (P < .05). The messenger RNA level of osteogenic genes increased significantly in the MTA and BA groups compared with controls (P < .05). However, although the messenger RNA level of osteogenic genes increased in the BD group, there was no statistically significant difference compared with controls. MTA, BA, and BD led to an increase in ALP staining compared with controls. CONCLUSIONS: In conclusion, MTA, BA, and BD have effects on osteoblast differentiation in mesenchymal stem cells, suggesting that these cements may be useful for root-end filling material.
INTRODUCTION: Because a root-end filling material comes into contact with the surrounding cells or tissues, understanding the cell-material interfacial activity is important. Thus, the purpose of this study was to assess the biocompatibility of 3 endodontic bioactive cements (MTA [Dentsply, Tulsa, OK], Bioaggregate [BA; Innovative Bioceramix, Vancouver, BC, Canada], and Biodentine [BD; Septodont, St Maur des Fosses, France]) and to investigate the effect of cements on the differentiation of mesenchymal stem cells. METHODS: Cell viability, mineralization, and differentiation were evaluated using an 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay and alkaline phosphatase (ALP) staining. The expressions of ALP, osteocalcin, and bone sialoprotein at the gene level were detected by reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. RESULTS: Cell viability of BD in concentrations of 1, 1/2, and 1/4 was significantly lower than MTA and BA (P < .05). There was no statistically significant difference in cell viability between materials in concentrations of 1/10 and 1/50 (P < .05). The messenger RNA level of osteogenic genes increased significantly in the MTA and BA groups compared with controls (P < .05). However, although the messenger RNA level of osteogenic genes increased in the BD group, there was no statistically significant difference compared with controls. MTA, BA, and BD led to an increase in ALP staining compared with controls. CONCLUSIONS: In conclusion, MTA, BA, and BD have effects on osteoblast differentiation in mesenchymal stem cells, suggesting that these cements may be useful for root-end filling material.
Authors: Hend Okasha; Ashraf M Abu-Seida; Ahmed A Hashem; Salma H El Ashry; Mohamed M Nagy Journal: Int J Exp Pathol Date: 2022-04-01 Impact factor: 2.793
Authors: Frangis Nikfarjam; Kim Beyer; Anke König; Matthias Hofmann; Manuel Butting; Eva Valesky; Stefan Kippenberger; Roland Kaufmann; Detlef Heidemann; August Bernd; Nadja Nicole Zöller Journal: PLoS One Date: 2016-12-09 Impact factor: 3.240
Authors: Alejandro Victoria-Escandell; José Santiago Ibañez-Cabellos; Sergio Bañuls-Sánchez de Cutanda; Ester Berenguer-Pascual; Jesús Beltrán-García; Eva García-López; Federico V Pallardó; José Luis García-Giménez; Antonio Pallarés-Sabater; Ignacio Zarzosa-López; Manuel Monterde Journal: Stem Cells Int Date: 2017-05-24 Impact factor: 5.443