Literature DB >> 2506479

Immunological and chemical characterization of hamster brain polyribosomes-cytomatrix complexes.

I Bouhtiauy1, Y Choukri, C Turpin, D Gauthier.   

Abstract

We have studied the cytoskeletal nature of a brain subcellular fraction previously shown to contain polyribosomes. We have identified the major proteins of this fraction by electrophoretic comparison to a standard cytoskeletal fraction and by immunodetection. These methods have shown the presence of actin, glial fibrillary acidic protein, and neurofilament triplet proteins. We have also studied the effect of various ions and nonionic detergents on the stability of this structure. It was stable in presence of Triton X-100 up to 2% but disrupted by 200 mM K+ acetate.

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Year:  1989        PMID： 2506479     DOI： 10.1007/bf00964872

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  29 in total

1.  Neurofilament disguise, destruction and discipline.

Authors:  D S Gilbert; B J Newby
Journal:  Nature       Date:  1975-08-14       Impact factor: 49.962

2.  Efficacy of RNase inhibitors during brain polysome isolation.

Authors:  D Gauthier; M R Ven Murthy
Journal:  Neurochem Res       Date:  1987-04       Impact factor: 3.996

3.  Isolation of hamster brain polyribosomes-cytoskeleton complexes.

Authors:  H Lequang; D Gauthier
Journal:  Neurochem Res       Date:  1989-03       Impact factor: 3.996

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Association of an aminoacyl-tRNA synthetase complex and of phenylalanyl-tRNA synthetase with the cytoskeletal framework fraction from mammalian cells.

Authors:  M Mirande; D Le Corre; D Louvard; H Reggio; J P Pailliez; J P Waller
Journal:  Exp Cell Res       Date:  1985-01       Impact factor: 3.905

6.  The cytoskeletal framework of sea urchin eggs and embryos: developmental changes in the association of messenger RNA.

Authors:  R T Moon; R F Nicosia; C Olsen; M B Hille; W R Jeffery
Journal:  Dev Biol       Date:  1983-02       Impact factor: 3.582

7.  On the association of mRNA with the cytoskeleton in uninfected and adenovirus-infected human KB cells.

Authors:  W J van Venrooij; P T Sillekens; C A van Eekelen; R J Reinders
Journal:  Exp Cell Res       Date:  1981-09       Impact factor: 3.905

8.  Association of maternal and newly synthesized ribosomes with membranous noncytoskeletal structures in Xenopus laevis embryonic cells.

Authors:  K Shiokawa; K Tashiro; Y Misumi
Journal:  J Exp Zool       Date:  1985-08

9.  A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

Authors:  M S Blake; K H Johnston; G J Russell-Jones; E C Gotschlich
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

10.  Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.

Authors:  M Cervera; G Dreyfuss; S Penman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582
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