Literature DB >> 2498751

Isolation of hamster brain polyribosomes-cytoskeleton complexes.

H Lequang1, D Gauthier.   

Abstract

We have developed a method for the isolation of a brain subcellular fraction enriched in both highly aggregated polyribosomes and cytoskeletal proteins. This method is based on gentle homogenization of brain tissue and low speed centrifugation. The mechanism of association of polyribosomes to cytoskeletal structures has been studied by in vitro treatment of this fraction with polyribosome-disaggregating agents. RNase and EDTA, while succeeding in completely disrupting them into monosomes or subunits, did not release them from cytoskeleton. Puromycin showed no noticeable effect.

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Year:  1989        PMID： 2498751     DOI： 10.1007/bf00971317

Source DB:  PubMed          Journal:  Neurochem Res        ISSN： 0364-3190            Impact factor:   3.996


  19 in total

1.  Efficacy of RNase inhibitors during brain polysome isolation.

Authors:  D Gauthier; M R Ven Murthy
Journal:  Neurochem Res       Date:  1987-04       Impact factor: 3.996

2.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Association of an aminoacyl-tRNA synthetase complex and of phenylalanyl-tRNA synthetase with the cytoskeletal framework fraction from mammalian cells.

Authors:  M Mirande; D Le Corre; D Louvard; H Reggio; J P Pailliez; J P Waller
Journal:  Exp Cell Res       Date:  1985-01       Impact factor: 3.905

4.  The cytoskeletal framework of sea urchin eggs and embryos: developmental changes in the association of messenger RNA.

Authors:  R T Moon; R F Nicosia; C Olsen; M B Hille; W R Jeffery
Journal:  Dev Biol       Date:  1983-02       Impact factor: 3.582

5.  On the association of mRNA with the cytoskeleton in uninfected and adenovirus-infected human KB cells.

Authors:  W J van Venrooij; P T Sillekens; C A van Eekelen; R J Reinders
Journal:  Exp Cell Res       Date:  1981-09       Impact factor: 3.905

6.  Association of maternal and newly synthesized ribosomes with membranous noncytoskeletal structures in Xenopus laevis embryonic cells.

Authors:  K Shiokawa; K Tashiro; Y Misumi
Journal:  J Exp Zool       Date:  1985-08

7.  Cytochalasin releases mRNA from the cytoskeletal framework and inhibits protein synthesis.

Authors:  D A Ornelles; E G Fey; S Penman
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272

8.  Cytoplasmic microtubules are essential for the formation of membrane-bound polyribosomes.

Authors:  P R Walker; J F Whitfield
Journal:  J Biol Chem       Date:  1985-01-25       Impact factor: 5.157

9.  Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells.

Authors:  M Cervera; G Dreyfuss; S Penman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

10.  Effect of viral infection on host protein synthesis and mRNA association with the cytoplasmic cytoskeletal structure.

Authors:  A M Bonneau; A Darveau; N Sonenberg
Journal:  J Cell Biol       Date:  1985-04       Impact factor: 10.539
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  3 in total

Review 1.  Interaction between mRNA, ribosomes and the cytoskeleton.

Authors:  J E Hesketh; I F Pryme
Journal:  Biochem J       Date:  1991-07-01       Impact factor: 3.857

2.  Immunological and chemical characterization of hamster brain polyribosomes-cytomatrix complexes.

Authors:  I Bouhtiauy; Y Choukri; C Turpin; D Gauthier
Journal:  Neurochem Res       Date:  1989-07       Impact factor: 3.996

Review 3.  Translation and the cytoskeleton: a mechanism for targeted protein synthesis.

Authors:  J Hesketh
Journal:  Mol Biol Rep       Date:  1994-05       Impact factor: 2.316
  3 in total

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