BACKGROUND: NAD(+) is an endogenous analyte and is unstable during blood sample collection, both of which present obstacles for quantitation. Moreover, current procedures for NAD(+) sample collection require onsite treatment with strong acid to stabilize the NAD(+) in mouse blood cells. RESULTS: NAD(+) can be stabilized by addition of acid before the frozen mouse blood sample was thawed. A simple sample collection procedure was proposed to facilitate the analysis of NAD(+) in mouse blood and tissue samples. A LC-MS/MS method was developed for quantifying NAD(+) in mouse blood and various tissue samples. The described method was used to measure endogenous NAD(+) levels in mouse blood following oral administration of the nicotinamide phosphoribosyltransferase inhibitor GNE-617. CONCLUSION: This study presents a suitable assay and sample collection procedure for high throughput screening of NAD(+) samples in preclinical discovery studies.
BACKGROUND:NAD(+) is an endogenous analyte and is unstable during blood sample collection, both of which present obstacles for quantitation. Moreover, current procedures for NAD(+) sample collection require onsite treatment with strong acid to stabilize the NAD(+) in mouse blood cells. RESULTS:NAD(+) can be stabilized by addition of acid before the frozen mouse blood sample was thawed. A simple sample collection procedure was proposed to facilitate the analysis of NAD(+) in mouse blood and tissue samples. A LC-MS/MS method was developed for quantifying NAD(+) in mouse blood and various tissue samples. The described method was used to measure endogenous NAD(+) levels in mouse blood following oral administration of the nicotinamide phosphoribosyltransferase inhibitor GNE-617. CONCLUSION: This study presents a suitable assay and sample collection procedure for high throughput screening of NAD(+) samples in preclinical discovery studies.
Authors: Krish Chandrasekaran; Neda Najimi; Avinash R Sagi; Sushuma Yarlagadda; Mohammad Salimian; Muhammed Ikbal Arvas; Ahmad F Hedayat; Yanni Kevas; Anand Kadakia; James W Russell Journal: Int J Mol Sci Date: 2022-04-28 Impact factor: 6.208
Authors: Puneet Bagga; Hari Hariharan; Neil E Wilson; Joanne C Beer; Russell T Shinohara; Mark A Elliott; Joseph A Baur; Francesco M Marincola; Walter R Witschey; Mohammad Haris; John A Detre; Ravinder Reddy Journal: Magn Reson Med Date: 2019-09-10 Impact factor: 4.668
Authors: Krish Chandrasekaran; Joungil Choi; Muhammed Ikbal Arvas; Mohammad Salimian; Sujal Singh; Su Xu; Rao P Gullapalli; Tibor Kristian; James William Russell Journal: Int J Mol Sci Date: 2020-05-26 Impact factor: 5.923
Authors: Philipp Brunnbauer; Annekatrin Leder; Can Kamali; Kaan Kamali; Eriselda Keshi; Katrin Splith; Simon Wabitsch; Philipp Haber; Georgi Atanasov; Linda Feldbrügge; Igor M Sauer; Johann Pratschke; Moritz Schmelzle; Felix Krenzien Journal: Sci Rep Date: 2018-10-31 Impact factor: 4.379