Jian Li1, Geng Guo2, Jin Li3, Jiehe Hao4, Jianjun Zhang5, Yongping Guo5, Hui Yu5. 1. Department of Neurosurgery, Changzhi City People's Hospital, Changzhi, Shanxi Province, China. Electronic address: 13935535420@139.com. 2. Department of Neurosurgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China. Electronic address: guogeng973@163.com. 3. Center of Health Examination, Changzhi City People's Hospital, Changzhi, Shanxi Province, China. 4. Department of Neurosurgery, The First Hospital, Shanxi Medical University, Taiyuan, Shanxi Province, China. 5. Department of Neurosurgery, Changzhi City People's Hospital, Changzhi, Shanxi Province, China.
Abstract
BACKGROUND: The proto-oncogene dishevelled (Dvl) is a critical component of the Wnt/β-catenin signaling pathway, and its elevated expression in various tumor types is associated with malignancy. However, a role for Dvl in glioma has not been explored. MATERIALS AND METHODS: To determine whether Dvl expression is elevated in human glioma, we examined the protein levels in 67 human glioma samples and 3 normal brain specimens by Western blotting and immunohistochemistry. To investigate a possible association of Dvl with the malignant phenotype in glioma, the correlation of the Dvl immunoreactivity score (IRS) with β-catenin IRS, the tumor proliferation index (PI), and tumor invasion index (II) were determined for each sample. RESULTS: The Dvl IRS, β-catenin IRS, PI, and II increased significantly with the pathologic grade of glioma (P <0.001) with average scores of 3.46 ± 3.45, 3.92 ± 3.28, 30.93 ± 17.92, and 20.43 ± 11.79, respectively. Furthermore, the PI and II were significantly higher for the Dvl-positive group than the Dvl-negative group (P <0.001). Correlation analysis demonstrated that β-catenin IRS, PI, and II were positively correlated with Dvl IRS. CONCLUSIONS: Dvl overexpression may contribute to the malignant proliferation and invasion of human glioma.
BACKGROUND: The proto-oncogene dishevelled (Dvl) is a critical component of the Wnt/β-catenin signaling pathway, and its elevated expression in various tumor types is associated with malignancy. However, a role for Dvl in glioma has not been explored. MATERIALS AND METHODS: To determine whether Dvl expression is elevated in humanglioma, we examined the protein levels in 67 humanglioma samples and 3 normal brain specimens by Western blotting and immunohistochemistry. To investigate a possible association of Dvl with the malignant phenotype in glioma, the correlation of the Dvl immunoreactivity score (IRS) with β-catenin IRS, the tumor proliferation index (PI), and tumor invasion index (II) were determined for each sample. RESULTS: The Dvl IRS, β-catenin IRS, PI, and II increased significantly with the pathologic grade of glioma (P <0.001) with average scores of 3.46 ± 3.45, 3.92 ± 3.28, 30.93 ± 17.92, and 20.43 ± 11.79, respectively. Furthermore, the PI and II were significantly higher for the Dvl-positive group than the Dvl-negative group (P <0.001). Correlation analysis demonstrated that β-catenin IRS, PI, and II were positively correlated with Dvl IRS. CONCLUSIONS:Dvl overexpression may contribute to the malignant proliferation and invasion of humanglioma.