M T Ruf1, A Andreoli, P Itin, G Pluschke, P Schmid. 1. Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002, Basel, Switzerland; University of Basel, Basel, Switzerland.
Abstract
BACKGROUND: The ribosomal protein S6 is part of the translation machinery and is activated by phosphorylation via the mammalian target of rapamycin pathway, which is activated in psoriatic skin. OBJECTIVES: To investigate which S6 sites are phosphorylated in psoriasis and atopic dermatitis (AD), and to study whether S6 phosphorylation is associated with inflammation and/or keratinocyte hyperproliferation. METHODS: Healthy skin and skin lesions of patients with psoriasis and AD were investigated by immunostaining using antibodies that stain proliferating cells, leucocytes and distinct phosphorylated sites of S6. RESULTS: All psoriasis and AD lesions revealed abnormal S6 phosphorylation in the epidermis. The extent of S6 phosphorylation was diverse, generally stronger in psoriasis and correlated, in both diseases, with inflammation. S6 showed differential phosphorylation in distinct epidermal layers, which was most pronounced in hyperproliferative regions. CONCLUSIONS: Differential S6 phosphorylation may have a role in abnormal keratinocyte proliferation/differentiation.
BACKGROUND: The ribosomal protein S6 is part of the translation machinery and is activated by phosphorylation via the mammalian target of rapamycin pathway, which is activated in psoriatic skin. OBJECTIVES: To investigate which S6 sites are phosphorylated in psoriasis and atopic dermatitis (AD), and to study whether S6 phosphorylation is associated with inflammation and/or keratinocyte hyperproliferation. METHODS: Healthy skin and skin lesions of patients with psoriasis and AD were investigated by immunostaining using antibodies that stain proliferating cells, leucocytes and distinct phosphorylated sites of S6. RESULTS: All psoriasis and AD lesions revealed abnormal S6 phosphorylation in the epidermis. The extent of S6 phosphorylation was diverse, generally stronger in psoriasis and correlated, in both diseases, with inflammation. S6 showed differential phosphorylation in distinct epidermal layers, which was most pronounced in hyperproliferative regions. CONCLUSIONS: Differential S6 phosphorylation may have a role in abnormal keratinocyte proliferation/differentiation.
Authors: Jean Christopher Chamcheu; Maria-Ines Chaves-Rodriquez; Vaqar M Adhami; Imtiaz A Siddiqui; Gary S Wood; B Jack Longley; Hasan Mukhtar Journal: Acta Derm Venereol Date: 2016-08-23 Impact factor: 4.437
Authors: Xin Ma; Yi Ru; Ying Luo; Le Kuai; Qi-Long Chen; Yun Bai; Ye-Qiang Liu; Jia Chen; Yue Luo; Jian-Kun Song; Mi Zhou; Bin Li Journal: Front Cell Dev Biol Date: 2022-07-07
Authors: William R Swindell; Henriette A Remmer; Mrinal K Sarkar; Xianying Xing; Drew H Barnes; Liza Wolterink; John J Voorhees; Rajan P Nair; Andrew Johnston; James T Elder; Johann E Gudjonsson Journal: Genome Med Date: 2015-08-04 Impact factor: 11.117