Lars Henning Schmidt1, Dennis Görlich, Tilmann Spieker, Christian Rohde, Martin Schuler, Michael Mohr, Julia Humberg, Tim Sauer, Nils H Thoenissen, Andreas Huge, Reinhard Voss, Alessandro Marra, Andreas Faldum, Carsten Müller-Tidow, Wolfgang E Berdel, Rainer Wiewrodt. 1. *Department of Medicine A, Hematology, Oncology and Pulmonology, University Hospital Muenster; †Institute of Biostatistics and Clinical Research, Westfaelische Wilhelms-Universitaet Muenster; ‡Gerhard-Domagk Institute of Pathology, University Hospital Muenster, Muenster; §Department of Medical Oncology, West German Cancer Center, University Hospital Essen, University Duisburg-Essen, Essen; ‖German Cancer Consortium (DKTK), Heidelberg; ¶Integrated Functional Genomics, Interdisciplinary Center for Clinical Research, University of Muenster, Muenster; #Chest Surgery, Klinikum St. Georg, Ostercappeln, Ostercappeln, Germany; and **Department of Medicine, Hematology and Oncology, University of Halle, Halle, Germany.
Abstract
INTRODUCTION: Apoptosis is a crucial pathway in tumor growth and metastatic development. Apoptotic proteins regulate the underlying molecular cascades and are thought to modulate the tumor response to chemotherapy and radiation. However, the prognostic value of the expression of apoptosis regulators in localized non-small-cell lung cancer (NSCLC) is still unclear. METHODS: We investigated the protein expression of apoptosis regulators Bcl-2, Bcl-xl, Mcl-1, and pp32/PHAPI, and the expression of the lncRNA MALAT-1 in tumor samples from 383 NSCLC patients (median age: 65.6 years; 77.5% male; paraffin-embedded tissue microarrays). For statistical analysis correlation tests, Log rank tests and Cox proportional hazard models were applied. RESULTS: Tumor histology was significantly associated with the expression of Bcl-2, Bcl-xl and Mcl-1 (all p < 0.001). Among the tested apoptotic markers only Bcl-2 demonstrated prognostic impact (hazard ratio = 0.64, p = 0.012). For NSCLC patients with non-adenocarcinoma histology, Bcl-2 expression was associated with increased overall survival (p = 0.036). Besides tumor histology, prognostic impact of Bcl-2 was also found to depend on MALAT-1 lncRNA expression. Gene expression analysis of A549 adenocarcinoma cells with differential MALAT-1 lncRNA expression demonstrated an influence on the expression of Bcl-2 and its interacting proteins. CONCLUSIONS: Bcl-2 expression was specifically associated with superior prognosis in localized NSCLC. An interaction of Bcl-2 with MALAT-1 lncRNA expression was revealed, which merits further investigation for risk prediction in resectable NSCLC patients.
INTRODUCTION: Apoptosis is a crucial pathway in tumor growth and metastatic development. Apoptotic proteins regulate the underlying molecular cascades and are thought to modulate the tumor response to chemotherapy and radiation. However, the prognostic value of the expression of apoptosis regulators in localized non-small-cell lung cancer (NSCLC) is still unclear. METHODS: We investigated the protein expression of apoptosis regulators Bcl-2, Bcl-xl, Mcl-1, and pp32/PHAPI, and the expression of the lncRNA MALAT-1 in tumor samples from 383 NSCLCpatients (median age: 65.6 years; 77.5% male; paraffin-embedded tissue microarrays). For statistical analysis correlation tests, Log rank tests and Cox proportional hazard models were applied. RESULTS:Tumor histology was significantly associated with the expression of Bcl-2, Bcl-xl and Mcl-1 (all p < 0.001). Among the tested apoptotic markers only Bcl-2 demonstrated prognostic impact (hazard ratio = 0.64, p = 0.012). For NSCLCpatients with non-adenocarcinoma histology, Bcl-2 expression was associated with increased overall survival (p = 0.036). Besides tumor histology, prognostic impact of Bcl-2 was also found to depend on MALAT-1 lncRNA expression. Gene expression analysis of A549 adenocarcinoma cells with differential MALAT-1 lncRNA expression demonstrated an influence on the expression of Bcl-2 and its interacting proteins. CONCLUSIONS:Bcl-2 expression was specifically associated with superior prognosis in localized NSCLC. An interaction of Bcl-2 with MALAT-1 lncRNA expression was revealed, which merits further investigation for risk prediction in resectable NSCLCpatients.
Authors: C Jungnickel; L H Schmidt; L Bittigkoffer; L Wolf; A Wolf; F Ritzmann; A Kamyschnikow; C Herr; M D Menger; T Spieker; R Wiewrodt; R Bals; C Beisswenger Journal: Oncogene Date: 2017-03-27 Impact factor: 9.867
Authors: Gang Wang; Liang Xiao; Fen Wang; Jing Yang; Li Yang; Ye Zhao; Wensen Jin Journal: Radiat Environ Biophys Date: 2019-06-15 Impact factor: 1.925
Authors: Ghattas El Bassit; Rekha S Patel; Gay Carter; Vyshakh Shibu; Achintya A Patel; Shijie Song; Michel Murr; Denise R Cooper; Paula C Bickford; Niketa A Patel Journal: Endocrinology Date: 2017-01-01 Impact factor: 4.736