Literature DB >> 2503434

A strategy for producing single-stranded DNA in the polymerase chain reaction. A direct method for genomic sequencing.

M Kreitman1, L F Landweber.   

Abstract

A strategy for synthesizing single-stranded DNA by the polymerase chain reaction method is explored and two protocols are developed. DNA produced with these methods can be sequenced using standard 35S or 32P dideoxy sequencing protocols. The methods allow rapid genetic screening and population genetic analysis of sequence variation.

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Year:  1989        PMID： 2503434     DOI： 10.1016/0735-0651(89)90021-6

Source DB:  PubMed          Journal:  Gene Anal Tech        ISSN： 0735-0651


  10 in total

1.  Inferring the evolutionary histories of the Adh and Adh-dup loci in Drosophila melanogaster from patterns of polymorphism and divergence.

Authors:  M Kreitman; R R Hudson
Journal:  Genetics       Date:  1991-03       Impact factor: 4.562

2.  An rbcL sequence from a Miocene Taxodium (bald cypress).

Authors:  P S Soltis; D E Soltis; C J Smiley
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

3.  Regulation of intestinal lactase in adult hypolactasia.

Authors:  M Lloyd; G Mevissen; M Fischer; W Olsen; D Goodspeed; M Genini; W Boll; G Semenza; N Mantei
Journal:  J Clin Invest       Date:  1992-02       Impact factor: 14.808

4.  Thermal cycle dideoxy DNA sequencing.

Authors:  B E Slatko
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

5.  Instability of simple sequence DNA in Saccharomyces cerevisiae.

Authors:  S T Henderson; T D Petes
Journal:  Mol Cell Biol       Date:  1992-06       Impact factor: 4.272

6.  Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusion.

Authors:  E A Vallen; M A Hiller; T Y Scherson; M D Rose
Journal:  J Cell Biol       Date:  1992-06       Impact factor: 10.539

7.  The product of the Drosophila melanogaster segment polarity gene armadillo is highly conserved in sequence and expression in the housefly Musca domestica.

Authors:  M Peifer; E Wieschaus
Journal:  J Mol Evol       Date:  1993-03       Impact factor: 2.395

8.  Structure of the chromosomal gene and cDNAs coding for lactase-phlorizin hydrolase in humans with adult-type hypolactasia or persistence of lactase.

Authors:  W Boll; P Wagner; N Mantei
Journal:  Am J Hum Genet       Date:  1991-05       Impact factor: 11.025

9.  Neutral and non-neutral evolution of Drosophila mitochondrial DNA.

Authors:  D M Rand; M Dorfsman; L M Kann
Journal:  Genetics       Date:  1994-11       Impact factor: 4.562

10.  Evidence for a role of the Drosophila melanogaster suppressor of sable gene in the pre-mRNA splicing pathway.

Authors:  R A Fridell; L L Searles
Journal:  Mol Cell Biol       Date:  1994-01       Impact factor: 4.272
  10 in total

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