Karolina Kedzierska1, Katarzyna Sporniak-Tutak2, Agnieszka Kolasa3, Lesze Domański4, Maciej Domański4, Krzysztof Sindrewicz2, Tomasz Smektała2, Joanna Bober5, Krzysztof Safranow6, Bogumiła Osekowska4, Joanna Kabat-Koperska4, Irena Baranowska-Bosiacka6, Mirosław Parafiniuk7, Elźbieta Urasińska8, Kazimierz Ciechanowski4. 1. Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland. karolina.kedzierska@interia.pl. 2. Department of Dental Surgery, Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland. 3. 3epartment of Histology and mbryology Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland. 4. Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University, Szczecin, Poland. 5. Department of Medical Chemistry Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland. 6. Department of Biochemistry Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland. 7. Department of Forensic Medicine Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland. 8. Department of Pathomorphology Pomeranian Medical University Pomeranian Medical University, Szczecin, Poland.
Abstract
AIM: To analyse the impact of the most commonly used immunosuppressive drugs on the occurrence of apoptosis in the native kidneys of Wistar rats. METHOD: The study involved 36 rats. the animals being grouped according to the immunosuppressive regimen used (tacrolimus, mycophenolate mofetil, cyclosporine A, rapamycin and prednisone). The rats in all study groups were treated with a 3-drug protocol for 6 months. The medication dose was adjusted based on available literature data. No drugs were administered to the control group. The rats were then killed. Autopsies of all animals were performed and the kidneys were isolated for histopathology (HE + PAS). To assess cell apoptosis the TUNEL reaction was performed. Blood trough levels of immunosuppressive drugs as well as the parameters of peripheral blood were determined. RESULTS: 1. In rats treated with cyclosporine A distal nephron tubules were characterised by more pronounced apoptosis. 2. In tacrolimus-treated rats a lower intensity of apoptosis was found in the distal tubules. 3. In rapamycin-treated rats the apoptosis was inhibited both in the distal and proximal nephron tubules. 4. In MMF treated rats intense apoptosis was observed in the proximal nephron tubules. 5. There were no significant changes in renal histopathology (HE + PAS). CONCLUSIONS: The apoptosis in nephron tubules caused by immunosuppressive therapy is not accompanied by any histopathological changes (eg fibrosis, inflammation, tubular atrophy, vacuolation of the tubular cells) in light microscopy.
AIM: To analyse the impact of the most commonly used immunosuppressive drugs on the occurrence of apoptosis in the native kidneys of Wistar rats. METHOD: The study involved 36 rats. the animals being grouped according to the immunosuppressive regimen used (tacrolimus, mycophenolate mofetil, cyclosporine A, rapamycin and prednisone). The rats in all study groups were treated with a 3-drug protocol for 6 months. The medication dose was adjusted based on available literature data. No drugs were administered to the control group. The rats were then killed. Autopsies of all animals were performed and the kidneys were isolated for histopathology (HE + PAS). To assess cell apoptosis the TUNEL reaction was performed. Blood trough levels of immunosuppressive drugs as well as the parameters of peripheral blood were determined. RESULTS: 1. In rats treated with cyclosporine A distal nephron tubules were characterised by more pronounced apoptosis. 2. In tacrolimus-treated rats a lower intensity of apoptosis was found in the distal tubules. 3. In rapamycin-treated rats the apoptosis was inhibited both in the distal and proximal nephron tubules. 4. In MMF treated rats intense apoptosis was observed in the proximal nephron tubules. 5. There were no significant changes in renal histopathology (HE + PAS). CONCLUSIONS: The apoptosis in nephron tubules caused by immunosuppressive therapy is not accompanied by any histopathological changes (eg fibrosis, inflammation, tubular atrophy, vacuolation of the tubular cells) in light microscopy.
Authors: Anna Surówka; Kamila Szumilas; Aleksandra Wilk; Kamila Misiakiewicz-Has; Kazimierz Ciechanowski; Karolina Kędzierska-Kapuza Journal: Int J Environ Res Public Health Date: 2022-05-24 Impact factor: 4.614
Authors: Mirza Saim Baig; Agnieszka Kolasa-Wołosiuk; Anna Pilutin; Krzysztof Safranow; Irena Baranowska-Bosiacka; Joanna Kabat-Koperska; Barbara Wiszniewska Journal: Int J Environ Res Public Health Date: 2019-05-16 Impact factor: 3.390
Authors: Karolina Kędzierska; Krzysztof Sindrewicz; Katarzyna Sporniak-Tutak; Joanna Bober; Małgorzata Stańczyk-Dunaj; Barbara Dołęgowska; Robert Kaliszczak; Jerzy Sieńko; Joanna Kabat-Koperska; Edyta Gołembiewska; Kazimierz Ciechanowski Journal: Med Sci Monit Date: 2016-06-11
Authors: Aleksandra Wilk; Dagmara Szypulska-Koziarska; Karolina Kędzierska-Kapuza; Jerzy Sieńko; Agnieszka Kolasa-Wołosiuk; Kazimierz Ciechanowski; Barbara Wiszniewska Journal: Med Sci Monit Date: 2019-11-02