Li Wang1, Pingwei Zhao1, Liang Ma1, Yuxing Shan1, Zhenyu Jiang1, Juan Wang1, Yanfang Jiang2. 1. From the Key Laboratory for Zoonosis Research, Ministry of Education; and the Department of Rheumatism, The First Hospital, Jilin University, Changchun, China.L. Wang, MD; P. Zhao, MD; L. Ma, MD; Y. Shan, MD; J. Wang, MD; Y. Jiang, MD, PhD, Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital; Z. Jiang, Department of Rheumatism, The First Hospital, Jilin University. 2. From the Key Laboratory for Zoonosis Research, Ministry of Education; and the Department of Rheumatism, The First Hospital, Jilin University, Changchun, China.L. Wang, MD; P. Zhao, MD; L. Ma, MD; Y. Shan, MD; J. Wang, MD; Y. Jiang, MD, PhD, Key Laboratory for Zoonosis Research, Ministry of Education, The First Hospital; Z. Jiang, Department of Rheumatism, The First Hospital, Jilin University. yanfangjiang@hotmail.com.
Abstract
OBJECTIVE: To elucidate the potential role of follicular helper T cells (TFH) and interleukin 10 (IL-10)+ B cells in the development of systemic lupus erythematosus (SLE). METHODS: The numbers of peripheral blood CD27+, CD38+, CD86+, CD95+, IL-10+ B cells, and inducible T cell costimulator (ICOS)+, programmed death-1 (PD-1)+, IL-21+, CXCR5+CD4+ TFH-like cells were examined in 23 patients with new onset SLE and 20 healthy controls (HC). RESULTS: In comparison with HC, significantly reduced numbers of CD19+ and IL-10+ B cells, but increased numbers of CD27(high), CD86+, CD95+ B cells, CXCR5+CD4+, ICOS+, PD-1+, and IL-21+ TFH-like cells were detected, which were accompanied by higher levels of serum IL-21, but lower levels of IL-10 in the patients. Treatment with anti-SLE therapy modulated the imbalance of different subsets of B and TFH-like cells. The levels of serum IL-21 and IL-10 were positively correlated with the numbers of CD4+CXCR5+ TFH-like and CD19+CD5+CD1d+ B cells in the patients, respectively. The numbers of CD27(high) B cells were correlated positively with IL-21+ TFH-like cells, but negatively with IL-10+ B cells. The values of SLE Disease Activity Index, C3, and erythrocyte sedimentation rate were correlated positively with serum IL-21, but negatively with IL-10 in those patients. CONCLUSION: Our data indicate that the imbalance of IL-21+ TFH-like, CD27(high), and IL-10+ B cells may be associated with the pathogenesis of SLE, and levels of serum IL-21 and IL-10 may be valuable for evaluating disease activity in SLE.
OBJECTIVE: To elucidate the potential role of follicular helper T cells (TFH) and interleukin 10 (IL-10)+ B cells in the development of systemic lupus erythematosus (SLE). METHODS: The numbers of peripheral blood CD27+, CD38+, CD86+, CD95+, IL-10+ B cells, and inducible T cell costimulator (ICOS)+, programmed death-1 (PD-1)+, IL-21+, CXCR5+CD4+ TFH-like cells were examined in 23 patients with new onset SLE and 20 healthy controls (HC). RESULTS: In comparison with HC, significantly reduced numbers of CD19+ and IL-10+ B cells, but increased numbers of CD27(high), CD86+, CD95+ B cells, CXCR5+CD4+, ICOS+, PD-1+, and IL-21+ TFH-like cells were detected, which were accompanied by higher levels of serum IL-21, but lower levels of IL-10 in the patients. Treatment with anti-SLE therapy modulated the imbalance of different subsets of B and TFH-like cells. The levels of serum IL-21 and IL-10 were positively correlated with the numbers of CD4+CXCR5+ TFH-like and CD19+CD5+CD1d+ B cells in the patients, respectively. The numbers of CD27(high) B cells were correlated positively with IL-21+ TFH-like cells, but negatively with IL-10+ B cells. The values of SLE Disease Activity Index, C3, and erythrocyte sedimentation rate were correlated positively with serum IL-21, but negatively with IL-10 in those patients. CONCLUSION: Our data indicate that the imbalance of IL-21+ TFH-like, CD27(high), and IL-10+ B cells may be associated with the pathogenesis of SLE, and levels of serum IL-21 and IL-10 may be valuable for evaluating disease activity in SLE.
Entities:
Keywords:
B CELLS; FOLLICULAR HELPER T-LIKE CELLS; INTERLEUKIN 10; INTERLEUKIN 21; REGULATORY B CELL; SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: Vaishali R Moulton; Abel Suarez-Fueyo; Esra Meidan; Hao Li; Masayuki Mizui; George C Tsokos Journal: Trends Mol Med Date: 2017-06-13 Impact factor: 11.951
Authors: Maria P Karampetsou; Denis Comte; Abel Suárez-Fueyo; Eri Katsuyama; Nobuya Yoshida; Michihito Kono; Vasileios C Kyttaris; George C Tsokos Journal: Arthritis Rheumatol Date: 2019-01 Impact factor: 10.995
Authors: Seung-Chul Choi; Tarun E Hutchinson; Anton A Titov; Howard R Seay; Shiwu Li; Todd M Brusko; Byron P Croker; Shahram Salek-Ardakani; Laurence Morel Journal: J Immunol Date: 2016-06-13 Impact factor: 5.422
Authors: M Alahgholi-Hajibehzad; H Durmuş; F Aysal; Y Gülşen-Parman; P Oflazer; F Deymeer; G Saruhan-Direskeneli Journal: Clin Exp Immunol Date: 2017-07-28 Impact factor: 4.330