Literature DB >> 25017901

Evaluation of fluorescent stains for visualizing extracellular DNA in biofilms.

Mira Okshevsky1, Rikke Louise Meyer2.   

Abstract

Here we determine an optimal technique for the visualization of extracellular DNA in bacterial biofilms using the fluorescent eDNA stain TOTO-1 and the counterstain SYTO 60. This technique allows for more sensitive eDNA visualization than other fluorescent staining methods currently in use.
Copyright © 2014 Elsevier B.V. All rights reserved.

Keywords:  Biofilm; Extracellular DNA; Fluorescent staining; Imaging; eDNA

Mesh：

Substances：

Year:  2014        PMID： 25017901     DOI： 10.1016/j.mimet.2014.07.010

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  27 in total

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