Blendi Ura1, Giordana Feriotto2, Lorenzo Monasta3, Sabrine Bilel4, Marina Zweyer5, Claudio Celeghini6. 1. Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy. Electronic address: blendi.ura2006@libero.it. 2. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy. 3. Institute for Maternal and Child Health, IRCCS "Burlo Garofolo", Trieste, Italy. 4. Higher Institute of Technological Studies, University of Bizerte, Bizerte, Tunisia. 5. Department of Medical, Surgical and Health Sciences, University of Trieste, Trieste, Italy. 6. Department of Life Sciences, University of Trieste, Trieste, Italy.
Abstract
OBJECTIVE: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24). MATERIALS AND METHODS: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. RESULTS: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. CONCLUSION: Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
OBJECTIVE: To identify microRNAs (miRNAs) differentially expressed at early stages of gestation (12-14 weeks) in the serum of pregnant women, who later developed severe preeclampsia (sPE) in the third trimester of pregnancy (n = 24) compared to women with normal pregnancy (n = 24). MATERIALS AND METHODS: Sera from 12-14-week-gestation whole blood were subjected to microarray analysis with TaqMan Low Density Array chips (human microRNA panel V3.0), and to quantitative real-time polymerase chain reaction. RESULTS: By using the TaqMan Low Density Array chip technology, 19 mature miRNAs appeared differentially expressed in the group of women who later developed sPE as compared to normal women. The expression of four miRNAs (miR-1233, miR-520, miR-210, miR-144) was validated by quantitative real-time polymerase chain reaction analysis. MiR-1233 was the most overexpressed in the serum of women who later developed sPE. CONCLUSION: Circulating miRNAs deserve further investigation in order to explore their potential role in the pathogenesis of preeclampsia. In particular, miR-1233 might represent a potential marker of early sPE.
Authors: Mohammad L Rahman; Liming Liang; Linda Valeri; Li Su; Zhaozhong Zhu; Shangzhi Gao; Golam Mostofa; Qazi Qamruzzaman; Russ Hauser; Andrea Baccarelli; David C Christiani Journal: Epigenetics Date: 2018-08-13 Impact factor: 4.528
Authors: Laura María Rodríguez Santa; Laura Yuriko González Teshima; Jose Vicente Forero Forero; Andres Orlando Castillo Giraldo Journal: J Pregnancy Date: 2015-10-13