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Literature DB >> 24997370

Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.

Liang Wang¹, Yujing Han¹, Shuo Zhou¹, Xiyun Guan².

Abstract

A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.

Entities: Chemical Disease Mutation Species

Keywords: Biosensors; HIV-1 protease; Nanopore; Peptide; Proteolysis

Mesh：

Substances：

Year: 2014 PMID： 24997370 PMCID： PMC4412846 DOI： 10.1016/j.bios.2014.06.041

Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618

34 in total

1. Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.

Authors: W Vercoutere; S Winters-Hilt; H Olsen; D Deamer; D Haussler; M Akeson
Journal: Nat Biotechnol Date: 2001-03 Impact factor: 54.908

2. Single-molecule covalent chemistry with spatially separated reactants.

Authors: Tudor Luchian; Seong-Ho Shin; Hagan Bayley
Journal: Angew Chem Int Ed Engl Date: 2003-08-18 Impact factor: 15.336

3. Protein biosensors based on biofunctionalized conical gold nanotubes.

Authors: Zuzanna Siwy; Lacramioara Trofin; Punit Kohli; Lane A Baker; Christina Trautmann; Charles R Martin
Journal: J Am Chem Soc Date: 2005-04-13 Impact factor: 15.419

4. Real-time monitoring of peptide cleavage using a nanopore probe.

Authors: Qitao Zhao; Ranulu Samanthi S de Zoysa; Deqiang Wang; Dilani A Jayawardhana; Xiyun Guan
Journal: J Am Chem Soc Date: 2009-05-13 Impact factor: 15.419

5. Nanopore detection of 8-oxo-7,8-dihydro-2'-deoxyguanosine in immobilized single-stranded DNA via adduct formation to the DNA damage site.

Authors: Anna E P Schibel; Na An; Qian Jin; Aaron M Fleming; Cynthia J Burrows; Henry S White
Journal: J Am Chem Soc Date: 2010-12-07 Impact factor: 15.419

6. Stochastic study of the effect of ionic strength on noncovalent interactions in protein pores.

Authors: Qitao Zhao; Dilani A Jayawardhana; Xiyun Guan
Journal: Biophys J Date: 2007-11-09 Impact factor: 4.033

7. Nanopore detection of copper ions using a polyhistidine probe.

Authors: Guihua Wang; Liang Wang; Yujing Han; Shuo Zhou; Xiyun Guan
Journal: Biosens Bioelectron Date: 2013-10-23 Impact factor: 10.618

8. Electrochemical enzyme-linked immunosorbent assay featuring proximal reagent generation: detection of human immunodeficiency virus antibodies in clinical samples.

Authors: Alyajahan Bhimji; Alexandre A Zaragoza; Ludovic S Live; Shana O Kelley
Journal: Anal Chem Date: 2013-07-03 Impact factor: 6.986

Review 9. Reflections on 30 years of AIDS.

Authors: Kevin M De Cock; Harold W Jaffe; James W Curran
Journal: Emerg Infect Dis Date: 2011-06 Impact factor: 6.883

10. Rapid quantification of the latent reservoir for HIV-1 using a viral outgrowth assay.

Authors: Gregory M Laird; Evelyn E Eisele; S Alireza Rabi; Jun Lai; Stanley Chioma; Joel N Blankson; Janet D Siliciano; Robert F Siliciano
Journal: PLoS Pathog Date: 2013-05-30 Impact factor: 6.823

14 in total

1. Salt-Mediated Nanopore Detection of ADAM-17.

Authors: Xiaohan Chen; Youwen Zhang; Golbarg Mohammadi Roozbahani; Xiyun Guan
Journal: ACS Appl Bio Mater Date: 2018-12-24

2. A Nanopore Approach for Analysis of Caspase-7 Activity in Cell Lysates.

Authors: Bach Pham; Scott J Eron; Maureen E Hill; Xin Li; Monifa A Fahie; Jeanne A Hardy; Min Chen
Journal: Biophys J Date: 2019-08-02 Impact factor: 4.033

Review 3. Nanopore Sensing.

Authors: Wenqing Shi; Alicia K Friedman; Lane A Baker
Journal: Anal Chem Date: 2016-11-18 Impact factor: 6.986

Real-time label-free measurement of HIV-1 protease activity by nanopore analysis.

1. Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.

2. Single-molecule covalent chemistry with spatially separated reactants.

3. Protein biosensors based on biofunctionalized conical gold nanotubes.

4. Real-time monitoring of peptide cleavage using a nanopore probe.

5. Nanopore detection of 8-oxo-7,8-dihydro-2'-deoxyguanosine in immobilized single-stranded DNA via adduct formation to the DNA damage site.

6. Stochastic study of the effect of ionic strength on noncovalent interactions in protein pores.

7. Nanopore detection of copper ions using a polyhistidine probe.

8. Electrochemical enzyme-linked immunosorbent assay featuring proximal reagent generation: detection of human immunodeficiency virus antibodies in clinical samples.

Review 9. Reflections on 30 years of AIDS.

10. Rapid quantification of the latent reservoir for HIV-1 using a viral outgrowth assay.

1. Salt-Mediated Nanopore Detection of ADAM-17.

2. A Nanopore Approach for Analysis of Caspase-7 Activity in Cell Lysates.

Review 3. Nanopore Sensing.

4. Nano-channel of viral DNA packaging motor as single pore to differentiate peptides with single amino acid difference.

5. Label-free nanopore single-molecule measurement of trypsin activity.

6. Chemically functionalized conical PET nanopore for protein detection at the single-molecule level.

7. Simultaneous detection of multiple proteases using a non-array nanopore platform.

8. Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices.

9. Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease.

10. Monitoring tetracycline through a solid-state nanopore sensor.