Tao Huang1, Jianqin Sun2, Yanqiu Chen2, Hua Xie2, Danfeng Xu2, Jinyan Huang3, Duo Li4. 1. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China; Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. 2. Clinical Nutrition Center, Huadong Hospital, Fudan University, Shanghai, China. 3. Department of Biostatistics, Harvard School of Public Health, Boston, MA, USA. 4. Department of Food Science and Nutrition, Zhejiang University, Hangzhou, China. Electronic address: duoli@zju.edu.cn.
Abstract
OBJECTIVE: The aim of this study was to examine the association of the genetic variants in the fatty acid desaturase (FADS) gene cluster with erythrocyte phospholipid fatty acids (PLFA), and their relation to risk for type 2 diabetes mellitus (T2DM) in Han Chinese. METHODS: Seven hundred and fifty-eight patients with T2DM and 400 healthy individuals were recruited. The erythrocyte PLFA and single-nucleotide polymorphism were determined by standard method. RESULTS: Minor allele homozygotes and heterozygotes of rs174575 and rs174537 had lower PL 20:4 ω-6 levels in healthy individuals. Minor allele homozygotes and heterozygotes of rs174455 in FADS3 gene had lower levels of 22:5 ω-3, 20:4 ω-6, and Δ5desaturase activity in patients with T2DM. Erythrocyte membrane PL 18:3 ω-3 (P for trend = 0.002), 22:5 ω-3 (P for trend < 0.001), ω-3 polyunsaturated fatty acid (P for trend < 0.001), and ω-3:ω-6 (P for trend < 0.001) were significantly inversely associated with risk for T2DM. CONCLUSION: Genetic variants in the FADS gene cluster are associated with altered erythrocyte PLFAs. High levels of PL 18:3 ω-3, 22:5 ω-3, and total ω-3 polyunsaturated fatty acid were associated with low risk for T2DM.
OBJECTIVE: The aim of this study was to examine the association of the genetic variants in the fatty acid desaturase (FADS) gene cluster with erythrocyte phospholipid fatty acids (PLFA), and their relation to risk for type 2 diabetes mellitus (T2DM) in Han Chinese. METHODS: Seven hundred and fifty-eight patients with T2DM and 400 healthy individuals were recruited. The erythrocyte PLFA and single-nucleotide polymorphism were determined by standard method. RESULTS: Minor allele homozygotes and heterozygotes of rs174575 and rs174537 had lower PL 20:4 ω-6 levels in healthy individuals. Minor allele homozygotes and heterozygotes of rs174455 in FADS3 gene had lower levels of 22:5 ω-3, 20:4 ω-6, and Δ5desaturase activity in patients with T2DM. Erythrocyte membrane PL 18:3 ω-3 (P for trend = 0.002), 22:5 ω-3 (P for trend < 0.001), ω-3 polyunsaturated fatty acid (P for trend < 0.001), and ω-3:ω-6 (P for trend < 0.001) were significantly inversely associated with risk for T2DM. CONCLUSION: Genetic variants in the FADS gene cluster are associated with altered erythrocyte PLFAs. High levels of PL 18:3 ω-3, 22:5 ω-3, and total ω-3 polyunsaturated fatty acid were associated with low risk for T2DM.
Authors: Yao Hu; Huaixing Li; Ling Lu; Ani Manichaikul; Jingwen Zhu; Yii-Der I Chen; Liang Sun; Shuang Liang; David S Siscovick; Lyn M Steffen; Michael Y Tsai; Stephen S Rich; Rozenn N Lemaitre; Xu Lin Journal: Hum Mol Genet Date: 2016-01-06 Impact factor: 6.150
Authors: Andrea de la Garza Puentes; Rosa Montes Goyanes; Aida Maribel Chisaguano Tonato; Francisco José Torres-Espínola; Miriam Arias García; Leonor de Almeida; María Bonilla Aguirre; Marcela Guerendiain; Ana Isabel Castellote Bargalló; Maite Segura Moreno; Luz García-Valdés; Cristina Campoy; M Carmen Lopez-Sabater Journal: PLoS One Date: 2017-06-09 Impact factor: 3.240