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Literature DB >> 24984963

The application of KillerRed for acute protein inactivation in living cells.

Abstract

Generating loss of protein function is a powerful investigatory tool particularly if carried out on a physiologically relevant timescale in a live-cell fluorescent imaging experiment. KillerRed mediated chromophore assisted light inactivation (CALI) uses genetic encoding for specificity and light for acute inactivation that can also be spatially restricted. This unit provides protocols for setting up and carrying out properly controlled KillerRed experiments during live-cell imaging of cultured cells.

Entities: CellLine Chemical Disease Gene Species

Keywords: CALI; KillerRed; ROS; acute inactivation; protein inactivation

Mesh：

Substances：

Year: 2014 PMID： 24984963 PMCID： PMC5333992 DOI： 10.1002/0471142956.cy1235s69

Source DB: PubMed Journal: Curr Protoc Cytom ISSN： 1934-9297

23 in total

1. Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I.

Authors: Kurt W Marek; Graeme W Davis
Journal: Neuron Date: 2002-12-05 Impact factor: 17.173

2. Irradiation-induced protein inactivation reveals Golgi enzyme cycling to cell periphery.

Authors: Timothy Jarvela; Adam D Linstedt
Journal: J Cell Sci Date: 2012-03-15 Impact factor: 5.285

3. A genetically encoded photosensitizer.

Authors: Maria E Bulina; Dmitriy M Chudakov; Olga V Britanova; Yurii G Yanushevich; Dmitry B Staroverov; Tatyana V Chepurnykh; Ekaterina M Merzlyak; Maria A Shkrob; Sergey Lukyanov; Konstantin A Lukyanov
Journal: Nat Biotechnol Date: 2005-12-20 Impact factor: 54.908

4. Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.

Authors: Sergei Pletnev; Nadya G Gurskaya; Nadya V Pletneva; Konstantin A Lukyanov; Dmitri M Chudakov; Vladimir I Martynov; Vladimir O Popov; Mikhail V Kovalchuk; Alexander Wlodawer; Zbigniew Dauter; Vladimir Pletnev
Journal: J Biol Chem Date: 2009-09-08 Impact factor: 5.157

5. Diffusion pathways of oxygen species in the phototoxic fluorescent protein KillerRed.

Authors: Arijit Roy; Philippe Carpentier; Dominique Bourgeois; Martin Field
Journal: Photochem Photobiol Sci Date: 2010-08-31 Impact factor: 3.982

6. Structural basis for the phototoxicity of the fluorescent protein KillerRed.

Authors: Philippe Carpentier; Sebastien Violot; Laurent Blanchoin; Dominique Bourgeois
Journal: FEBS Lett Date: 2009-07-30 Impact factor: 4.124

7. Intracellular delivery and activation of the genetically encoded photosensitizer Killer Red by quantum dots encapsulated in polymeric micelles.

Authors: Muthunarayanan Muthiah; Seung-Hwan Park; Md Nurunnabi; Jooyoung Lee; Yong-Kyu Lee; Hansoo Park; Byeong-Il Lee; Jung-Joon Min; In-Kyu Park
Journal: Colloids Surf B Biointerfaces Date: 2014-01-17 Impact factor: 5.268

Review 8. Molecular effectors of multiple cell death pathways initiated by photodynamic therapy.

Authors: Esther Buytaert; Michael Dewaele; Patrizia Agostinis
Journal: Biochim Biophys Acta Date: 2007-07-06

9. SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation.

Authors: Kiwamu Takemoto; Tomoki Matsuda; Naoki Sakai; Donald Fu; Masanori Noda; Susumu Uchiyama; Ippei Kotera; Yoshiyuki Arai; Masataka Horiuchi; Kiichi Fukui; Tokiyoshi Ayabe; Fuyuhiko Inagaki; Hiroshi Suzuki; Takeharu Nagai
Journal: Sci Rep Date: 2013 Impact factor: 4.379