Literature DB >> 24982556

Evaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays.

Hwi Won Seo1, Kiwon Han1, Yeonsu Oh1, Ikjae Kang1, Changhoon Park1, Hye Eun Joo1, Sung-Hoon Kim1, Bog-Hieu Lee1, Chanhee Chae1.   

Abstract

The objective of the present study was to evaluate polyclonal- and monoclonal-antibody-based immunohistochemical (IHC) tests for the detection of 2 genotypes of Porcine circovirus type 2 (PCV2), a and b, in formalin-fixed, paraffin-embedded lymph-node tissue from pigs with experimental or natural postweaning multisystemic wasting syndrome and to compare the IHC results with those of in-situ hybridization (ISH) assays. The ISH assays proved more sensitive than the IHC tests for the detection of PCV2a and PCV2b. According to these findings, polyclonal-antibody-based IHC testing is the most practical routine diagnostic method for the detection of PCV2 regardless of genotype because IHC testing is less technically complex than ISH testing. However, ISH assays are useful to differentiate between PCV2a and PCV2b in surveillance programs for the monitoring of PCV2 in swine herds.

Entities:  

Mesh：

Substances：

Year:  2014        PMID： 24982556      PMCID： PMC4068416     

Source DB:  PubMed          Journal:  Can J Vet Res        ISSN： 0830-9000            Impact factor:   1.310


  16 in total

Review 1.  Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology.

Authors:  C Chae
Journal:  Vet J       Date:  2004-07       Impact factor: 2.688

2.  Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues.

Authors:  Duyeol Kim; Yooncheol Ha; Yeonsu Oh; Kiwon Han; Seung Ho Noh; Cheolwoo Kim; Sung-Hoon Kim; Chanhee Chae
Journal:  J Vet Diagn Invest       Date:  2010-03       Impact factor: 1.279

3.  The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada.

Authors:  Carl A Gagnon; Donald Tremblay; Peter Tijssen; Marie-Hélène Venne; Alain Houde; Seyyed Mehdy Elahi
Journal:  Can Vet J       Date:  2007-08       Impact factor: 1.008

4.  A comparison of virus isolation, polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine circovirus 2 and porcine parvovirus in experimentally and naturally coinfected pigs.

Authors:  Junghyun Kim; Chanhee Chae
Journal:  J Vet Diagn Invest       Date:  2004-01       Impact factor: 1.279

5.  Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins.

Authors:  M Fort; M Sibila; A Allepuz; E Mateu; F Roerink; J Segalés
Journal:  Vaccine       Date:  2008-01-07       Impact factor: 3.641

6.  Comparison of efficacy of commercial one dose and two dose PCV2 vaccines using a mixed PRRSV-PCV2-SIV clinical infection model 2-3-months post vaccination.

Authors:  T Opriessnig; A R Patterson; D M Madson; N Pal; P G Halbur
Journal:  Vaccine       Date:  2008-12-25       Impact factor: 3.641

7.  Comparative study of in situ hybridization and immunohistochemistry for the detection of porcine circovirus 2 in formalin-fixed, paraffin-embedded tissues.

Authors:  Duyeol Kim; Yooncheol Ha; Yong-Hoon Lee; Sungwon Chae; Kichan Lee; Kiwon Han; Junghyun Kim; Ju-Ho Lee; Sung-Hoon Kim; Kyu-Kye Hwang; Chanhee Chae
Journal:  J Vet Med Sci       Date:  2009-07       Impact factor: 1.267

8.  A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence.

Authors:  L Grau-Roma; E Crisci; M Sibila; S López-Soria; M Nofrarias; M Cortey; L Fraile; A Olvera; J Segalés
Journal:  Vet Microbiol       Date:  2007-09-19       Impact factor: 3.293

9.  Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.

Authors:  I E Vincent; C P Carrasco; B Herrmann; B M Meehan; G M Allan; A Summerfield; K C McCullough
Journal:  J Virol       Date:  2003-12       Impact factor: 5.103

10.  In vitro studies on the infection and replication of porcine circovirus type 2 in cells of the porcine immune system.

Authors:  D F Gilpin; K McCullough; B M Meehan; F McNeilly; I McNair; L S Stevenson; J C Foster; J A Ellis; S Krakowka; B M Adair; G M Allan
Journal:  Vet Immunol Immunopathol       Date:  2003-08-15       Impact factor: 2.046
View more
  2 in total

1.  Surface Display of porcine circovirus type 2 antigen protein cap on the spores of bacillus subtilis 168: An effective mucosal vaccine candidate.

Authors:  Weijie Li; Jianzhen Li; Xixi Dai; Minggang Liu; Abdul Khalique; Zhenghua Wang; Yan Zeng; Dongmei Zhang; Xueqin Ni; Dong Zeng; Bo Jing; Kangcheng Pan
Journal:  Front Immunol       Date:  2022-09-15       Impact factor: 8.786

2.  The level of decoy epitope in PCV2 vaccine affects the neutralizing activity of sera in the immunized animals.

Authors:  Junyeong Jin; Changhoon Park; Sun-Hee Cho; Junho Chung
Journal:  Biochem Biophys Res Commun       Date:  2018-01-31       Impact factor: 3.575
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.