Y J Xu1, Y Fan. 1. Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, 310022, China, xyj8288@hotmail.com.
Abstract
INTRODUCTION: Growing evidence indicates that microRNAs (miRNAs) play critical roles in cancer progression. However, the role of miR-215 or miR-192 in gastric carcinogenesis remains unclear at this point. MATERIALS AND METHODS: Quantitative real-time PCR analysis is used to detect the expression levels of miR-215 or -192. Matrigel invasion assay is used to explore the effects of miR-215 or -192 on gastric cancer cells invasion ability. Statistical analysis is carried out by SPSS 19.0 software. RESULTS: MiR-215/192 is significantly up-regulated in gastric cancer tissues from gastrectomy. The up-regulation of miR-215 or -192 was related with the clinical characteristics of gastric cancer, such as lymph node metastasis. Inhibition of miR-215/192 significantly suppressed gastric cancer cell invasion. Statistical analysis indicated that the expression of miR-215 or -192 may be a potential candidate biomarker for gastric cancer prognosis. CONCLUSIONS: These data suggest that frequently up-regulated miR-215/192 in gastric cancer may participate in gastric cancer progression.
INTRODUCTION: Growing evidence indicates that microRNAs (miRNAs) play critical roles in cancer progression. However, the role of miR-215 or miR-192 in gastric carcinogenesis remains unclear at this point. MATERIALS AND METHODS: Quantitative real-time PCR analysis is used to detect the expression levels of miR-215 or -192. Matrigel invasion assay is used to explore the effects of miR-215 or -192 on gastric cancer cells invasion ability. Statistical analysis is carried out by SPSS 19.0 software. RESULTS:MiR-215/192 is significantly up-regulated in gastric cancer tissues from gastrectomy. The up-regulation of miR-215 or -192 was related with the clinical characteristics of gastric cancer, such as lymph node metastasis. Inhibition of miR-215/192 significantly suppressed gastric cancer cell invasion. Statistical analysis indicated that the expression of miR-215 or -192 may be a potential candidate biomarker for gastric cancer prognosis. CONCLUSIONS: These data suggest that frequently up-regulated miR-215/192 in gastric cancer may participate in gastric cancer progression.
Authors: Sara A Georges; Matthew C Biery; Soo-Yeon Kim; Janell M Schelter; Jane Guo; Aaron N Chang; Aimee L Jackson; Michael O Carleton; Peter S Linsley; Michele A Cleary; B Nelson Chau Journal: Cancer Res Date: 2008-12-15 Impact factor: 12.701
Authors: Flavia Pichiorri; Sung-Suk Suh; Alberto Rocci; Luciana De Luca; Cristian Taccioli; Ramasamy Santhanam; Wenchao Zhou; Don M Benson; Craig Hofmainster; Hansjuerg Alder; Michela Garofalo; Gianpiero Di Leva; Stefano Volinia; Huey-Jen Lin; Danilo Perrotti; Michael Kuehl; Rami I Aqeilan; Antonio Palumbo; Carlo M Croce Journal: Cancer Cell Date: 2010-10-19 Impact factor: 31.743