Literature DB >> 2497937

Transformation methods for halophilic archaebacteria.

S W Cline1, W L Lam, R L Charlebois, L C Schalkwyk, W F Doolittle.   

Abstract

We present a practical description of polyethylene glycol mediated spheroplast transformation of Halobacterium halobium and Halobacterium volcanii. This method has been applied to phage DNA transfection, plasmid DNA transformation, and transformation with linear fragments of high molecular weight genomic DNA. Efficient spheroplast regeneration allows uncomplicated recovery of transformed progeny. Transformations can be performed equally well using fresh or frozen cell preparations. These methods should find application in molecular cloning, genetic fine mapping, and strain construction.

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Year:  1989        PMID： 2497937     DOI： 10.1139/m89-022

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  127 in total

1.  Car: a cytoplasmic sensor responsible for arginine chemotaxis in the archaeon Halobacterium salinarum.

Authors:  K F Storch; J Rudolph; D Oesterhelt
Journal:  EMBO J       Date:  1999-03-01       Impact factor: 11.598

2.  An improved transposon for the halophilic archaeon Haloarcula hispanica.

Authors:  W G Woods; K Ngui; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

3.  Propionyl coenzyme A (propionyl-CoA) carboxylase in Haloferax mediterranei: Indispensability for propionyl-CoA assimilation and impacts on global metabolism.

Authors:  Jing Hou; Hua Xiang; Jing Han
Journal:  Appl Environ Microbiol       Date:  2014-11-14       Impact factor: 4.792

4.  Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea.

Authors:  A Cohen; W L Lam; R L Charlebois; W F Doolittle; L C Schalkwyk
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

5.  Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea.

Authors:  Leanne M Timpson; Diya Alsafadi; Cillín Mac Donnchadha; Susan Liddell; Michael A Sharkey; Francesca Paradisi
Journal:  Extremophiles       Date:  2011-10-21       Impact factor: 2.395

6.  A plasmid vector with a selectable marker for halophilic archaebacteria.

Authors:  M L Holmes; M L Dyall-Smith
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

7.  ORF4 of the Temperate Archaeal Virus SNJ1 Governs the Lysis-Lysogeny Switch and Superinfection Immunity.

Authors:  Beibei Chen; Zhao Chen; Yuchen Wang; Han Gong; Linshan Sima; Jiao Wang; Shushan Ouyang; Wenqiang Gan; Mart Krupovic; Xiangdong Chen; Shishen Du
Journal:  J Virol       Date:  2020-07-30       Impact factor: 5.103

8.  Construction and characterization of a gradually inducible expression vector for Halobacterium salinarum, based on the kdp promoter.

Authors:  Dorthe Kixmüller; Jörg-Christian Greie
Journal:  Appl Environ Microbiol       Date:  2012-01-27       Impact factor: 4.792

9.  Essentiality of the glnA gene in Haloferax mediterranei: gene conversion and transcriptional analysis.

Authors:  V Rodríguez-Herrero; G Payá; V Bautista; A Vegara; M Cortés-Molina; M Camacho; J Esclapez; M J Bonete
Journal:  Extremophiles       Date:  2020-04-16       Impact factor: 2.395

10.  Analysis of proteasome-dependent proteolysis in Haloferax volcanii cells, using short-lived green fluorescent proteins.

Authors:  Christopher J Reuter; Julie A Maupin-Furlow
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792
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