Literature DB >> 2497707

Identification of the Butyrivibrio fibrisolvens xylosidase gene (xylB) coding region and its expression in Escherichia coli.

G W Sewell1, E A Utt, R B Hespell, K F Mackenzie, L O Ingram.   

Abstract

The gene encoding the principal Butyrivibrio fibrisolvens xylosidase (xylB) has been cloned and expressed in Escherichia coli under the control of the lac promoter. The coding region for this gene was localized within a 3.2-kilobase B. fibrisolvens DNA fragment in pUC18. A new protein band was observed in recombinant E. coli containing xylB. This protein (approximately 60,000 molecular weight) was presumed to be the xylosidase monomer. The optimal pH (5.5) and substrate range for the recombinant and native xylosidases appeared identical. Both enzymes hydrolyzed xylo-oligosaccharides with chain lengths of 2 to 5 and both were inactive on xylan.

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Year:  1989        PMID： 2497707      PMCID： PMC184106          DOI： 10.1128/aem.55.2.306-311.1989

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  16 in total

1.  Rate of isolated hemicellulose degradation and utilization by pure cultures of rumen bacteria.

Authors:  B A Dehority
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2.  Isolation and Some Properties of a beta-d-Xylosidase from Clostridium acetobutylicum ATCC 824.

Authors:  S F Lee; C W Forsberg
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792

3.  Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus.

Authors:  H Moriyama; E Fukusaki; J Cabrera Crespo; A Shinmyo; H Okada
Journal:  Eur J Biochem       Date:  1987-08-03

4.  Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens.

Authors:  M A Cotta; R B Hespell
Journal:  Appl Environ Microbiol       Date:  1986-07       Impact factor: 4.792

Review 5.  Microbial ecology and activities in the rumen: Part II.

Authors:  P N Hobson; R J Wallace
Journal:  Crit Rev Microbiol       Date:  1982-05       Impact factor: 7.624

6.  N-(1-naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process.

Authors:  M Bounias
Journal:  Anal Biochem       Date:  1980-08       Impact factor: 3.365

7.  Molecular cloning of a xylanase gene from Bacteroides succinogenes and its expression in Escherichia coli.

Authors:  A Sipat; K A Taylor; R Y Lo; C W Forsberg; P J Krell
Journal:  Appl Environ Microbiol       Date:  1987-03       Impact factor: 4.792

8.  Isolation of two beta-xylosidase genes of Bacillus pumilus and comparison of their gene products.

Authors:  W Panbangred; O Kawaguchi; T Tomita; A Shinmyo; H Okada
Journal:  Eur J Biochem       Date:  1984-01-16

9.  Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli.

Authors:  W Panbangred; T Kondo; S Negoro; A Shinmyo; H Okada
Journal:  Mol Gen Genet       Date:  1983

10.  Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.

Authors:  J E Clark-Curtiss; W R Jacobs; M A Docherty; L R Ritchie; R Curtiss
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490
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  9 in total

1.  Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities.

Authors:  E A Utt; C K Eddy; K F Keshav; L O Ingram
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

2.  Purification and Characterization of an alpha-l-Arabinofuranosidase from Butyrivibrio fibrisolvens GS113.

Authors:  R B Hespell; P J O'bryan
Journal:  Appl Environ Microbiol       Date:  1992-04       Impact factor: 4.792

3.  Cloning, sequencing, and expression of a xylanase gene from the anaerobic ruminal bacterium Butyrivibrio fibrisolvens.

Authors:  B M Mannarelli; S Evans; D Lee
Journal:  J Bacteriol       Date:  1990-08       Impact factor: 3.490

4.  Conjugal transfer of Tn916, Tn916 delta E, and pAM beta 1 from Enterococcus faecalis to Butyrivibrio fibrisolvens strains.

Authors:  R B Hespell; T R Whitehead
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

5.  Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger.

Authors:  M J Flipphi; M van Heuvel; P van der Veen; J Visser; L H de Graaff
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

6.  Cloning and sequencing of a Bacteroides ruminicola B(1)4 endoglucanase gene.

Authors:  O Matsushita; J B Russell; D B Wilson
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

7.  The xylan-degrading enzyme system of Talaromyces emersonii: novel enzymes with activity against aryl beta-D-xylosides and unsubstituted xylans.

Authors:  M G Tuohy; J Puls; M Claeyssens; M Vrsanská; M P Coughlan
Journal:  Biochem J       Date:  1993-03-01       Impact factor: 3.857

8.  Degradation and utilization by Butyrivibrio fibrisolvens H17c of xylans with different chemical and physical properties.

Authors:  R B Hespell; M A Cotta
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

Review 9.  Molecular cloning and comparative sequence analysis of fungal β-Xylosidases.

Authors:  Ghulam Mustafa; Sumaira Kousar; Muhammad Ibrahim Rajoka; Amer Jamil
Journal:  AMB Express       Date:  2016-04-14       Impact factor: 3.298
  9 in total

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