Adaliene Versiani Matos Ferreira1, Zélia Menezes-Garcia2, Jonas Baeta Viana3, Erica Guilhen Mário3, Leida Maria Botion3. 1. Department of Nutrition, Nursing School, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: Adaliene@gmail.com. 2. Department of Microbiology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. 3. Department of Physiology and Biophysics, Biological Sciences Institute, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Abstract
OBJECTIVE: Several metabolic pathways may be associated with obesity development and may be differentially modulated by dietary constituents. The aim of this study was to access the adipocyte metabolic pathways that lead to lipid accumulation in fat cells of mice fed a high-carbohydrate or high-fat diet. METHODS: Male Swiss mice ages 7 to 8 wk were fed a standard laboratory rodent diet-chow diet, a high-carbohydrate (HC) diet, or a high-fat (HF) diet for 8 wk. RESULTS: Animals fed the HC diet exhibited a high lipogenesis rate and lipoprotein lipase activity even in a fasted state. Peroxisome proliferator-activated receptor gamma and sterol regulatory element-binding proteins-1 mRNAs were increased in adipose tissue. The HF diet did not promote the lipogenic pathways but attenuated lipolytic activity and glucose uptake in adipocytes. CONCLUSION: The HC diet induces constitutive expression of lipogenic transcription factors during fasting, whereas the HF diet decreases lipolysis in adipocytes. Both pathways may contribute to increase fat stores and maintain an obese state.
OBJECTIVE: Several metabolic pathways may be associated with obesity development and may be differentially modulated by dietary constituents. The aim of this study was to access the adipocyte metabolic pathways that lead to lipid accumulation in fat cells of mice fed a high-carbohydrate or high-fat diet. METHODS: Male Swiss mice ages 7 to 8 wk were fed a standard laboratory rodent diet-chow diet, a high-carbohydrate (HC) diet, or a high-fat (HF) diet for 8 wk. RESULTS: Animals fed the HC diet exhibited a high lipogenesis rate and lipoprotein lipase activity even in a fasted state. Peroxisome proliferator-activated receptor gamma and sterol regulatory element-binding proteins-1 mRNAs were increased in adipose tissue. The HF diet did not promote the lipogenic pathways but attenuated lipolytic activity and glucose uptake in adipocytes. CONCLUSION: The HC diet induces constitutive expression of lipogenic transcription factors during fasting, whereas the HF diet decreases lipolysis in adipocytes. Both pathways may contribute to increase fat stores and maintain an obese state.
Authors: Ana Beatriz Farias de Souza; Máira Tereza Talma Chírico; Christiane Teixeira Cartelle; Guilherme de Paula Costa; André Talvani; Sílvia Dantas Cangussú; Rodrigo Cunha Alvim de Menezes; Frank Silva Bezerra Journal: Oxid Med Cell Longev Date: 2018-02-12 Impact factor: 6.543