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Literature DB >> 24968026

Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.

Ryan D Walters¹, David T McSwiggen¹, James A Goodrich¹, Jennifer F Kugel¹.

Abstract

The AP-1 family of transcriptional activators plays pivotal roles in regulating a wide range of biological processes from the immune response to tumorigenesis. Determining the roles of specific AP-1 dimers in cells, however, has remained challenging because common molecular biology techniques are unable to distinguish between the role of, for example, cJun/cJun homodimers versus cJun/cFos heterodimers. Here we used SELEX (systematic evolution of ligands by exponential enrichment) to identify and characterize DNA aptamers that are >100-fold more specific for binding cJun/cJun compared to cJun/cFos, setting the foundation to investigate the biological functions of different AP-1 dimer compositions.

Entities: Chemical

Mesh：

Substances：

Year: 2014 PMID： 24968026 PMCID： PMC4072727 DOI： 10.1371/journal.pone.0101015

Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240

32 in total

4. Subtractive Cell-SELEX Selection of DNA Aptamers Binding Specifically and Selectively to Hepatocellular Carcinoma Cells with High Metastatic Potential.

Authors: Hao Chen; Chun-Hui Yuan; Yi-Fei Yang; Chang-Qing Yin; Qing Guan; Fu-Bing Wang; Jian-Cheng Tu
Journal: Biomed Res Int Date: 2016-03-28 Impact factor: 3.411

4 in total

Selection and characterization of a DNA aptamer that can discriminate between cJun/cJun and cJun/cFos.

1. Mfold web server for nucleic acid folding and hybridization prediction.

2. c-Jun is a JNK-independent coactivator of the PU.1 transcription factor.

3. Thioaptamer decoy targeting of AP-1 proteins influences cytokine expression and the outcome of arenavirus infections.

Review 4. Aptamers as therapeutic and diagnostic agents.

5. VARNA: Interactive drawing and editing of the RNA secondary structure.

6. Probing TBP interactions in transcription initiation and reinitiation with RNA aptamers that act in distinct modes.

7. Promoter specificity and biological activity of tethered AP-1 dimers.

8. The C-terminal region of human NFATc2 binds cJun to synergistically activate interleukin-2 transcription.

9. An RNA aptamer that interferes with the DNA binding of the HSF transcription activator.

10. NFATc2 recruits cJun homodimers to an NFAT site to synergistically activate interleukin-2 transcription.

Review 1. Trends in the Design and Development of Specific Aptamers Against Peptides and Proteins.

2. Identification of Sequence-Selective Tyrosine Kinase Deoxyribozymes.

Review 3. Aptamers: Uptake mechanisms and intracellular applications.

4. Subtractive Cell-SELEX Selection of DNA Aptamers Binding Specifically and Selectively to Hepatocellular Carcinoma Cells with High Metastatic Potential.