Li-Xia Zeng1, Yong Tang2, Yun Ma1. 1. Department of Pathology, The First Affiliate Hospital of Guangxi Medical University No. 21, Shuangyong RD, Nanning 530021, China. 2. Departments of Urology Surgery, Cancer Hospital of Guangxi Medical University No. 71, Nanning 530021, China.
Abstract
UNLABELLED: To investigate the expression of Ataxin-3 in human gastric cancer tissues and cell lines, and explore its clinical pathologic significance. METHODS: The expression of Ataxin-3 in gastric cancer (n=536) and noncancerous gastric mucosa (n=312) was determined by immunohistochemistry and correlated to clinicopathologic features such as histologic differentiation and tumor size. The expression of Ataxin-3 protein in the human gastric cancer cell lines MKN45, SGC7901 and in normal human gastric epithelial cells (GES-1) was also evaluated by Western blot analysis. Quantitative real-time PCR was used to determine Ataxin-3 mRNA expression in human gastric cancer cell lines and tissues. RESULTS: The expression of Ataxin-3 protein was decreased in the gastric cancer compared to noncancerous gastric tissue, and correlated with tumor size, Lauren classification, histologic differentiation, and mutant p53 protein (P < 0.05). Similarly, Ataxin-3 mRNA expression was decreased in the gastric cancers compared to the noncancerous gastric tissue. Ataxin-3 protein and mRNA expression was lower in MKN45, SGC7901 cells than in the normal GES-1 cells. CONCLUSION: Decreased expression of Ataxin-3 may play an important role in gastric carcinogenesis and development of gastric cancer.
UNLABELLED: To investigate the expression of Ataxin-3 in humangastric cancer tissues and cell lines, and explore its clinical pathologic significance. METHODS: The expression of Ataxin-3 in gastric cancer (n=536) and noncancerous gastric mucosa (n=312) was determined by immunohistochemistry and correlated to clinicopathologic features such as histologic differentiation and tumor size. The expression of Ataxin-3 protein in the humangastric cancer cell lines MKN45, SGC7901 and in normal human gastric epithelial cells (GES-1) was also evaluated by Western blot analysis. Quantitative real-time PCR was used to determine Ataxin-3 mRNA expression in humangastric cancer cell lines and tissues. RESULTS: The expression of Ataxin-3 protein was decreased in the gastric cancer compared to noncancerous gastric tissue, and correlated with tumor size, Lauren classification, histologic differentiation, and mutant p53 protein (P < 0.05). Similarly, Ataxin-3 mRNA expression was decreased in the gastric cancers compared to the noncancerous gastric tissue. Ataxin-3 protein and mRNA expression was lower in MKN45, SGC7901 cells than in the normal GES-1 cells. CONCLUSION: Decreased expression of Ataxin-3 may play an important role in gastric carcinogenesis and development of gastric cancer.
Entities:
Keywords:
Ataxin-3; clinicopathologic features; gastric cancer
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