Literature DB >> 24948090

Higher order structure characterization of protein therapeutics by hydrogen/deuterium exchange mass spectrometry.

Richard Y-C Huang1, Guodong Chen.   

Abstract

Characterization of therapeutic drugs is a crucial step in drug development in the biopharmaceutical industry. Analysis of protein therapeutics is a challenging task because of the complexities associated with large molecular size and 3D structures. Recent advances in hydrogen/deuterium-exchange mass spectrometry (HDX-MS) have provided a means to assess higher-order structure of protein therapeutics in solution. In this review, the principles and procedures of HDX-MS for protein therapeutics characterization are presented, focusing on specific applications of epitope mapping for protein-protein interactions and higher-order structure comparison studies for conformational dynamics of protein therapeutics.

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Year:  2014        PMID： 24948090     DOI： 10.1007/s00216-014-7924-3

Source DB:  PubMed          Journal:  Anal Bioanal Chem        ISSN： 1618-2642            Impact factor:   4.142


  31 in total

1.  Conformational Assessment of Adnectin and Adnectin-Drug Conjugate by Hydrogen/Deuterium Exchange Mass Spectrometry.

Authors:  Richard Y-C Huang; Steven R O'Neil; Daša Lipovšek; Guodong Chen
Journal:  J Am Soc Mass Spectrom       Date:  2018-05-07       Impact factor: 3.109

Review 2.  Research advances in hydrogen-deuterium exchange mass spectrometry for protein epitope mapping.

Authors:  Haofeng Sun; Lingyun Ma; Leyu Wang; Peng Xiao; Hongmei Li; Min Zhou; Dewei Song
Journal:  Anal Bioanal Chem       Date:  2021-01-06       Impact factor: 4.142

3.  A Retrospective Evaluation of the Use of Mass Spectrometry in FDA Biologics License Applications.

Authors:  Sarah Rogstad; Anneliese Faustino; Ashley Ruth; David Keire; Michael Boyne; Jun Park
Journal:  J Am Soc Mass Spectrom       Date:  2016-11-21       Impact factor: 3.109

4.  Localized conformational interrogation of antibody and antibody-drug conjugates by site-specific carboxyl group footprinting.

Authors:  Lucy Yan Pan; Oscar Salas-Solano; John F Valliere-Douglass
Journal:  MAbs       Date:  2016-12-08       Impact factor: 5.857

5.  Deciphering Binding Interactions of IL-23R with HDX-MS: Mapping Protein and Macrocyclic Dodecapeptide Ligands.

Authors:  Cristina Sayago; Isabel C Gonzalez Valcarcel; Yuewei Qian; John Lee; Jorge Alsina-Fernandez; Nathan C Fite; Juan J Carrillo; Feiyu F Zhang; Michael J Chalmers; Jeffrey A Dodge; Howard Broughton; Alfonso Espada
Journal:  ACS Med Chem Lett       Date:  2018-08-01       Impact factor: 4.345

6.  Hydrogen/deuterium exchange mass spectrometry and computational modeling reveal a discontinuous epitope of an antibody/TL1A Interaction.

Authors:  Richard Y-C Huang; Stanley R Krystek; Nathan Felix; Robert F Graziano; Mohan Srinivasan; Achal Pashine; Guodong Chen
Journal:  MAbs       Date:  2017-11-14       Impact factor: 5.857

7.  Orthogonal Mass Spectrometry-Based Footprinting for Epitope Mapping and Structural Characterization: The IL-6 Receptor upon Binding of Protein Therapeutics.

Authors:  Ke Sherry Li; Guodong Chen; Jingjie Mo; Richard Y-C Huang; Ekaterina G Deyanova; Brett R Beno; Steve R O'Neil; Adrienne A Tymiak; Michael L Gross
Journal:  Anal Chem       Date:  2017-07-06       Impact factor: 6.986

8.  Characterization of ELISA Antibody-Antigen Interaction using Footprinting-Mass Spectrometry and Negative Staining Transmission Electron Microscopy.

Authors:  Margaret Lin; Denise Krawitz; Matthew D Callahan; Galahad Deperalta; Aaron T Wecksler
Journal:  J Am Soc Mass Spectrom       Date:  2018-03-06       Impact factor: 3.109

9.  Characterization of Aggregation Propensity of a Human Fc-Fusion Protein Therapeutic by Hydrogen/Deuterium Exchange Mass Spectrometry.

Authors:  Richard Y-C Huang; Roxana E Iacob; Stanley R Krystek; Mi Jin; Hui Wei; Li Tao; Tapan K Das; Adrienne A Tymiak; John R Engen; Guodong Chen
Journal:  J Am Soc Mass Spectrom       Date:  2016-08-15       Impact factor: 3.109

10.  Native State of Complement Protein C3d Analysed via Hydrogen Exchange and Conformational Sampling.

Authors:  Didier Devaurs; Malvina Papanastasiou; Dinler A Antunes; Jayvee R Abella; Mark Moll; Daniel Ricklin; John D Lambris; Lydia E Kavraki
Journal:  Int J Comput Biol Drug Des       Date:  2018-03-24
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