OBJECTIVE: The aim of this study was to analyse the expression of crucial genes in fatty acid metabolism in visceral (VAT) and subcutaneous (SAT) adipose tissue samples from morbidly obese women. METHODS: The VAT and SAT expression of key genes in 145 morbidly obese women (MO, BMI > 40 Kg/m(2) ) and 18 normal weight control women by RT-PCR and Western Blot was analyzed. RESULTS: In SAT, the expression levels of the genes related to lipogenesis and fatty acid oxidation were significantly lower in MO than in controls. In VAT, most of the lipogenic genes studied had similar expression levels in MO and control cohort. Regarding inflammation, IL6 was significantly higher in MO in both tissues whereas TNFα mRNA expression was significantly higher only in VAT. CONCLUSIONS: Our results indicate that in morbidly obese patients, lipogenesis and fatty acid oxidation are downregulated in SAT, whereas in VAT these pathways are almost unchanged. By contrast, inflammation is induced in both adipose tissues. It is hypothesized that, in this type of extreme obesity, SAT works to limit any further development of fat mass, decreasing the expression of lipogenic and FA oxidative genes whereas VAT depot might have lost this capability.
OBJECTIVE: The aim of this study was to analyse the expression of crucial genes in fatty acid metabolism in visceral (VAT) and subcutaneous (SAT) adipose tissue samples from morbidly obesewomen. METHODS: The VAT and SAT expression of key genes in 145 morbidly obesewomen (MO, BMI > 40 Kg/m(2) ) and 18 normal weight control women by RT-PCR and Western Blot was analyzed. RESULTS: In SAT, the expression levels of the genes related to lipogenesis and fatty acid oxidation were significantly lower in MO than in controls. In VAT, most of the lipogenic genes studied had similar expression levels in MO and control cohort. Regarding inflammation, IL6 was significantly higher in MO in both tissues whereas TNFα mRNA expression was significantly higher only in VAT. CONCLUSIONS: Our results indicate that in morbidly obesepatients, lipogenesis and fatty acid oxidation are downregulated in SAT, whereas in VAT these pathways are almost unchanged. By contrast, inflammation is induced in both adipose tissues. It is hypothesized that, in this type of extreme obesity, SAT works to limit any further development of fat mass, decreasing the expression of lipogenic and FA oxidative genes whereas VAT depot might have lost this capability.
Authors: Tyler A Bosch; Lisa Chow; Donald R Dengel; Susan J Melhorn; Mary Webb; Danielle Yancey; Holly Callahan; Mary Rosalyn B De Leon; Vidhi Tyagi; Ellen A Schur Journal: Metabolism Date: 2015-06-06 Impact factor: 8.694
Authors: Teresa Auguet; Alba Berlanga; Esther Guiu-Jurado; Salomé Martinez; José Antonio Porras; Gemma Aragonès; Fátima Sabench; Mercé Hernandez; Carmen Aguilar; Joan Josep Sirvent; Daniel Del Castillo; Cristóbal Richart Journal: Int J Mol Sci Date: 2014-12-02 Impact factor: 5.923
Authors: Esther Guiu-Jurado; Teresa Auguet; Alba Berlanga; Gemma Aragonès; Carmen Aguilar; Fàtima Sabench; Sandra Armengol; José Antonio Porras; Andreu Martí; Rosa Jorba; Mercè Hernández; Daniel del Castillo; Cristóbal Richart Journal: Int J Mol Sci Date: 2015-12-16 Impact factor: 5.923
Authors: Nicholas J Carruthers; Clarissa Strieder-Barboza; Joseph A Caruso; Carmen G Flesher; Nicki A Baker; Samuel A Kerk; Alexander Ky; Anne P Ehlers; Oliver A Varban; Costas A Lyssiotis; Carey N Lumeng; Paul M Stemmer; Robert W O'Rourke Journal: Sci Rep Date: 2021-08-30 Impact factor: 4.379