Kais Kasem1, Emily Sullivan1, Vinod Gopalan1, Ali Salajegheh1, Robert A Smith1, Alfred K-Y Lam2. 1. Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia. 2. Cancer Molecular Pathology of Griffith Health Institute, Griffith University, Gold Coast, Queensland, Australia. Electronic address: a.lam@griffith.edu.au.
Abstract
BACKGROUND: JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. METHODS: Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line. RESULTS: JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates. CONCLUSIONS: JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
BACKGROUND:JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. METHODS: Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line. RESULTS:JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates. CONCLUSIONS:JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
Authors: Vida Chitsazzadeh; Cristian Coarfa; Jennifer A Drummond; Tri Nguyen; Aaron Joseph; Suneel Chilukuri; Elizabeth Charpiot; Charles H Adelmann; Grace Ching; Tran N Nguyen; Courtney Nicholas; Valencia D Thomas; Michael Migden; Deborah MacFarlane; Erika Thompson; Jianjun Shen; Yoko Takata; Kayla McNiece; Maxim A Polansky; Hussein A Abbas; Kimal Rajapakshe; Adam Gower; Avrum Spira; Kyle R Covington; Weimin Xiao; Preethi Gunaratne; Curtis Pickering; Mitchell Frederick; Jeffrey N Myers; Li Shen; Hui Yao; Xiaoping Su; Ronald P Rapini; David A Wheeler; Ernest T Hawk; Elsa R Flores; Kenneth Y Tsai Journal: Nat Commun Date: 2016-08-30 Impact factor: 14.919