Sjoerd A M E G Timmermans 1 , Jan G M C Damoiseaux 2 , Petra T J Heerings-Rewinkel 2 , Rivka Ayalon 3 , Laurence H Beck 3 , Wolfgang Schlumberger 4 , David J Salant 3 , Pieter van Paassen 5 , Jan Willem Cohen Tervaert 6 Show Affiliations »
Abstract
OBJECTIVES: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IIF) and a Western blotting test (WB). METHODS: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. RESULTS: With similar specificity (≥ 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P < .001). CONCLUSIONS: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF. Copyright© by the American Society for Clinical Pathology.
OBJECTIVES: Autoantibodies against the M-type phospholipase A2 receptor 1 (anti-PLA2R1 ) have been demonstrated to be very specific for idiopathic membranous nephropathy (MN). We studied a novel enzyme-linked immunosorbent assay (ELISA) and compared results with results obtained using an indirect immunofluorescence (IIF) and a Western blotting test (WB). METHODS: One-hundred nine patients with idiopathic MN were recruited between November 1979 and March 2011. The control cohort comprised serum samples from patients with secondary MN (n = 16) and nephrotic controls (n = 17). The presence of anti-PLA2R1 in serum samples obtained at the time of renal biopsy was determined using ELISA, IIF, and WB. RESULTS: With similar specificity (≥ 97%), sensitivity varied from 68% (IIF) to 72% (ELISA, WB). Remarkably, patients who were seronegative for anti-PLA2R1 more often entered spontaneous remission (P = .038), whereas seropositive patients were more frequently treated with immunosuppressive agents (P < .001). CONCLUSIONS: ELISA performs excellently in differentiating idiopathic from secondary MN. Furthermore, ELISA shared high agreement with WB and IIF. Copyright© by the American Society for Clinical Pathology.
Entities: Disease
Gene
Species
Keywords:
ELISA; Membranous nephropathy; Nephrotic syndrome; Phospholipase A2 receptor 1; Sensitivity and specificity
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Year: 2014
PMID： 24926082 DOI： 10.1309/AJCP8QMOY5GLRSFP
Source DB: PubMed Journal: Am J Clin Pathol ISSN： 0002-9173 Impact factor: 2.493