Literature DB >> 2492093

Isolation of an IgH gene circular DNA clone from human bone marrow.

M Abe1, H Shiku.   

Abstract

Circular DNA was obtained from human bone marrow. Then a phage library was prepared and screened by use of two probes of the IgH gene; 5'-DHQ52, containing the 5' flanking region of DHQ52, and JH4.3, containing the sequence from JH3 to the 3' flanking region of JH6. One clone, HBMC-1, that was DHQ52+JH4.3- was obtained. HBMC-1 had the germline IgH region upstream of JH1 and the 3' flanking region of DXP1. A recombination signal sequence flanking the 5' side of the JH1 segment was attached to the recombination signal sequence flanking the 3' side of DXP1 forming a head-to-head structure of two 7mers with 10 nucleotides in-between. HBMC-1 is thus considered to be a circular DNA deleted as a consequence of DXP1-JH1 joining of the IgH gene.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2492093      PMCID： PMC331542          DOI： 10.1093/nar/17.1.163

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Isolation of an excision product of T-cell receptor alpha-chain gene rearrangements.

Authors:  S Fujimoto; H Yamagishi
Journal:  Nature       Date:  1987 May 21-27       Impact factor: 49.962

2.  Reciprocal recombination products of VK-JK joining reactions in human lymphoid cell lines.

Authors:  S M Deev; G Combriato; H G Klobeck; H G Zachau
Journal:  Nucleic Acids Res       Date:  1987-01-12       Impact factor: 16.971

3.  Direct evidence for chromosomal inversion during T-cell receptor beta-gene rearrangements.

Authors:  M Malissen; C McCoy; D Blanc; J Trucy; C Devaux; A M Schmitt-Verhulst; F Fitch; L Hood; B Malissen
Journal:  Nature       Date:  1986 Jan 2-8       Impact factor: 49.962

4.  T cell receptor beta gene sequences in the circular DNA of thymocyte nuclei: direct evidence for intramolecular DNA deletion in V-D-J joining.

Authors:  K Okazaki; D D Davis; H Sakano
Journal:  Cell       Date:  1987-05-22       Impact factor: 41.582

5.  Structure of the human immunoglobulin mu locus: characterization of embryonic and rearranged J and D genes.

Authors:  J V Ravetch; U Siebenlist; S Korsmeyer; T Waldmann; P Leder
Journal:  Cell       Date:  1981-12       Impact factor: 41.582

6.  Determination of nucleotide sequences in DNA.

Authors:  F Sanger
Journal:  Science       Date:  1981-12-11       Impact factor: 47.728

7.  At least five DH genes of human immunoglobulin heavy chains are encoded in 9-kilobase DNA fragments.

Authors:  Y Ichihara; M Abe; H Yasui; H Matsuoka; Y Kurosawa
Journal:  Eur J Immunol       Date:  1988-04       Impact factor: 5.532

8.  Human immunoglobulin D segments encoded in tandem multigenic families.

Authors:  U Siebenlist; J V Ravetch; S Korsmeyer; T Waldmann; P Leder
Journal:  Nature       Date:  1981-12-17       Impact factor: 49.962

9.  The use of chromosomal translocations to study human immunoglobulin gene organization: mapping DH segments within 35 kb of the C mu gene and identification of a new DH locus.

Authors:  L Buluwela; D G Albertson; P Sherrington; P H Rabbitts; N Spurr; T H Rabbitts
Journal:  EMBO J       Date:  1988-07       Impact factor: 11.598

10.  Thymocyte circular DNA excised from T cell receptor alpha-delta gene complex.

Authors:  K Okazaki; H Sakano
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
View more
  1 in total

1.  Nucleotide sequence of an immunoglobulin D-J gene fusion from a B-cell prolymphocytic leukaemia.

Authors:  M A Kennedy; P E Crossen
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.