Amita Goyal1, Arpna Srivastava, Ramanjit Sihota, Jasbir Kaur. 1. Department of Ocular Biochemistry, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences , New Delhi , India and.
Abstract
PURPOSE: The present study was designed to determine the levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and non-enzymatic antioxidants (vitamins C and E) in aqueous humor of primary open angle glaucoma (POAG) and primary angle closure glaucoma (PACG) patients. MATERIALS AND METHODS: In this study, aqueous humor of POAG (n = 30) and PACG (n = 30) patients was obtained. For control, aqueous humor of 30 age-matched cataract patients (n = 30) was collected. Activities of antioxidant enzymes and non-enzymatic antioxidants levels were measured spectrophotometrically. RESULTS: A significant increase in superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities was found in aqueous humor of POAG and PACG patients as compared to cataract patients (p < 0.001). No significant changes were observed in catalase activity. The levels of vitamins C and E were significantly lower in the aqueous humor of POAG and PACG as compared to cataract patients (p < 0.001). CONCLUSION: These results suggest that a significant increase in oxidative stress may play a role in the pathogenesis of POAG and PACG. Determination of oxidative stress in aqueous humor may help in understanding the course of this disease, and oxidative damage might be a relevant target for both prevention and therapy.
PURPOSE: The present study was designed to determine the levels of antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and non-enzymatic antioxidants (vitamins C and E) in aqueous humor of primary open angle glaucoma (POAG) and primary angle closure glaucoma (PACG) patients. MATERIALS AND METHODS: In this study, aqueous humor of POAG (n = 30) and PACG (n = 30) patients was obtained. For control, aqueous humor of 30 age-matched cataractpatients (n = 30) was collected. Activities of antioxidant enzymes and non-enzymatic antioxidants levels were measured spectrophotometrically. RESULTS: A significant increase in superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities was found in aqueous humor of POAG and PACG patients as compared to cataractpatients (p < 0.001). No significant changes were observed in catalase activity. The levels of vitamins C and E were significantly lower in the aqueous humor of POAG and PACG as compared to cataractpatients (p < 0.001). CONCLUSION: These results suggest that a significant increase in oxidative stress may play a role in the pathogenesis of POAG and PACG. Determination of oxidative stress in aqueous humor may help in understanding the course of this disease, and oxidative damage might be a relevant target for both prevention and therapy.
Entities:
Keywords:
Antioxidant enzymes; cataract; glaucoma; vitamin C; vitamin E
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