| Literature DB >> 24899653 |
Collette Fitzgerald1, Zheng Chao Tu2, Mary Patrick1, Tracy Stiles3, Andy J Lawson4, Monica Santovenia1, Maarten J Gilbert5,6, Marcel van Bergen7,5, Kevin Joyce1, Janet Pruckler1, Steven Stroika1, Birgitta Duim5,6, William G Miller8, Vladimir Loparev1, Jan C Sinnige9, Patricia I Fields1, Robert V Tauxe1, Martin J Blaser2, Jaap A Wagenaar7,5,6.
Abstract
A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.Entities:
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Year: 2014 PMID： 24899653 DOI： 10.1099/ijs.0.057778-0
Source DB: PubMed Journal: Int J Syst Evol Microbiol ISSN： 1466-5026 Impact factor: 2.747