OBJECT: The objective of this study was to determine the effects of a standardized fat rich meal and subsequent exercise on liver fat content by ¹H MRS and on liver adenosine triphosphate (ATP) content by ³¹P MRS in healthy subjects. MATERIALS AND METHODS: Hepatic ¹H and proton decoupled ³¹P MRS were performed on nine healthy subjects on a clinical 3.0 T MR imager three times during a day: after (1) an overnight fast, (2) a following standardized fat rich meal and (3) a subsequent exercise session. Blood parameters were followed during the day to serve as a reference to MRS. RESULTS: Liver fat content increased gradually over the day (p = 0.0001) with an overall increase of 30 %. Also γ-NTP changed significantly over the day (p = 0.005). γ-NTP/tP decreased by 9 % (p = 0.019, post hoc) from the postprandial to the post-exercise state. CONCLUSION: Our study shows that in vivo MRS can depict short lived physiological changes; entering of fat into liver cells and consumption of ATP during exercise can be measured non-invasively in healthy subjects. The physiological state may have an impact on fat and energy metabolite levels. Hepatic ¹H and ³¹P MRS studies should be performed under standardized conditions.
OBJECT: The objective of this study was to determine the effects of a standardized fat rich meal and subsequent exercise on liver fat content by ¹H MRS and on liver adenosine triphosphate (ATP) content by ³¹P MRS in healthy subjects. MATERIALS AND METHODS: Hepatic ¹H and proton decoupled ³¹P MRS were performed on nine healthy subjects on a clinical 3.0 T MR imager three times during a day: after (1) an overnight fast, (2) a following standardized fat rich meal and (3) a subsequent exercise session. Blood parameters were followed during the day to serve as a reference to MRS. RESULTS: Liver fat content increased gradually over the day (p = 0.0001) with an overall increase of 30 %. Also γ-NTP changed significantly over the day (p = 0.005). γ-NTP/tP decreased by 9 % (p = 0.019, post hoc) from the postprandial to the post-exercise state. CONCLUSION: Our study shows that in vivo MRS can depict short lived physiological changes; entering of fat into liver cells and consumption of ATP during exercise can be measured non-invasively in healthy subjects. The physiological state may have an impact on fat and energy metabolite levels. Hepatic ¹H and ³¹P MRS studies should be performed under standardized conditions.
Authors: Jochem R van Werven; Johannes M Hoogduin; Aart J Nederveen; Andre A van Vliet; Ewa Wajs; Petra Vandenberk; Erik S G Stroes; Jaap Stoker Journal: J Magn Reson Imaging Date: 2009-08 Impact factor: 4.813
Authors: Marek Chmelik; Michal Považan; Martin Krššák; Stephan Gruber; Martin Tkačov; Siegfried Trattnig; Wolfgang Bogner Journal: NMR Biomed Date: 2014-02-24 Impact factor: 4.044
Authors: Marzena Wylezinska; Jeremy F L Cobbold; Julie Fitzpatrick; Mark J W McPhail; Mary M E Crossey; Howard C Thomas; Joseph V Hajnal; William Vennart; I Jane Cox; Simon D Taylor-Robinson Journal: NMR Biomed Date: 2010-10-15 Impact factor: 4.044