Literature DB >> 24886980

Microarray-based resonance light scattering assay for detecting DNA methylation and human DNA methyltransferase simultaneously with high sensitivity.

Lan Ma1, Min Su, Tao Li, Zhenxin Wang.   

Abstract

A microarray-based resonance light scattering assay, with the combination of methylation-sensitive endonuclease and gold nanoparticle (GNP) probes, has been proposed to sensitively distinguish the DNA methylation level as low as 0.01% (10 pM methylated DNA in 100 nM total DNA) and detect human DNA methyltransferase 1 (Dnmt1) down to 0.1 U mL(-1).

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Year:  2014        PMID: 24886980     DOI: 10.1039/c4an00336e

Source DB:  PubMed          Journal:  Analyst        ISSN: 0003-2654            Impact factor:   4.616


  1 in total

1.  Ultrasensitive DNA methyltransferase activity sensing and inhibitor evaluation with highly photostable upconversion nanoparticle transducer.

Authors:  Mingmin Wu; Mengtian Zhang; Zhiwei Fan; Xinyue Qin; Xiaoxia Zhu; Haiwei Ji; Yuling Qin; Qi Wang; Li Wu
Journal:  Mikrochim Acta       Date:  2021-04-23       Impact factor: 5.833

  1 in total

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