Mineralizing surface is the main target of mechanical stimulation independent of age: 3D dynamic in vivo morphometry.

Mechanical loading can increase cortical bone mass by shifting the balance between bone formation and resorption towards increased formation. With advancing age resorption outpaces formation resulting in a net loss in cortical bone mass. How cortical bone (re)modeling - especially resorption - responds to mechanical loading with aging remains unclear. In this study, we investigated age-related changes in the modulation of cortical bone formation and resorption sites by mechanical loading. Using in vivo microCT we determined the kinetics of three dimensional formation and resorption parameters. To analyze age-associated adaptation, the left tibiae of young, adult and elderly female C57BL/6 mice were cyclically loaded for 2weeks. Our data showed that in the nonloaded limbs, cortical bone loss with age is the result of an imbalance of resorption to formation thickness, while the surface of resorption is comparable to formation. Loading has a much stronger effect on formation than on resorption; more specifically this effect is due to an increase in formation surface with mechanical stimulation. This is the only effect of loading which is conserved into old age. The resorption thickness is independent of loading in all age groups. Using this novel image analysis technique, we were able for the first time to quantify age-related changes in cortical (re)modeling and the adaptive capacity to mechanics. Most likely a therapy against age-related bone loss combining physical exercise and pharmaceuticals is most efficient if they each act on different parameters of the (re)modeling process. Despite some differences in skeletal aging between mice and humans, our results would suggest that physical exercise in old individuals can positively influence only the formation side of (re) modeling.