Literature DB >> 24878640

Generation of an infectious Negev virus cDNA clone.

Rodion V Gorchakov1, Robert B Tesh1, Scott C Weaver1, Farooq Nasar1.   

Abstract

The genus Negevirus consists of insect-only viruses isolated from mosquitoes and sandflies. Here, we report the successful construction of a full-length infectious cDNA clone of Negev virus (NEGV) strain M30957. Viral RNA was transcribed in vitro and virus was readily rescued with or without the use of a cap analogue. These results strongly suggest that NEGV, and likely other members within the genus, is a non-segmented, single-stranded, positive-sense RNA virus.
© 2014 The Authors.

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Year:  2014        PMID： 24878640      PMCID： PMC4135092          DOI： 10.1099/vir.0.066019-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  11 in total

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