Effect of antibiotics in controlling the production of cell wall degrading enzymes in vitro and disease development in vivo.

Production of cellulolytic, proteolytic and pectolytic enzymes by all the three isolates of A. niger was inhibited by all the tested antibiotics. Nystatin (500 ppm) could cause complete inhibition of PG enzyme production, whereas PMG production was checked by amoxicillin, nystatin, chloramphenicol and raficillin at 100 ppm concentration. All the antibiotics at 500 ppm concentration checked the cellulase production in all the three isolates of A. niger. Nystatin and raficillin (100 ppm) completely controlled the protease production in all the three isolates of A. niger. A positive correlation was observed with enzyme production and mycelial biomass in three isolates of A. niger, All the antibiotics at both the concentrations (100 and 500 ppm) could also check the rottening in all the three fruits in vivo conditions.