Literature DB >> 24862277

Measurement of fatty acid oxidation rates in animal tissues and cell lines.

Frank K Huynh1, Michelle F Green1, Timothy R Koves2, Matthew D Hirschey3.   

Abstract

While much oncological research has focused on metabolic shifts in glucose and amino acid oxidation, recent evidence suggests that fatty acid oxidation (FAO) may also play an important role in the metabolic reprogramming of cancer cells. Here, we present a simple method for measuring FAO rates using radiolabeled palmitate, common laboratory reagents, and standard supplies. This protocol is broadly applicable for measuring FAO rates in cultured cancer cells as well as in both malignant and nontransformed animal tissues.
© 2014 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Acid-soluble metabolite; Cell lysate; Radiolabeling; Xenografts; β-Oxidation CO(2)

Mesh：

Substances：

Year:  2014        PMID： 24862277      PMCID： PMC4154315          DOI： 10.1016/B978-0-12-416618-9.00020-0

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


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