Franciéli P Rozales1, Vanessa B Ribeiro1, Cibele M Magagnin2, Mariana Pagano1, Larissa Lutz3, Diego R Falci4, Adão Machado5, Afonso L Barth1, Alexandre P Zavascki6. 1. Programa de Pós Graduação em Ciências Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil. 2. Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, Brazil; Programa de Pós Graduação em Ciências Médicas, Faculdade de Medicina, UFRGS, Porto Alegre, Brazil. 3. Unidade de Microbiologia, Serviço de Patologia Clínica, HCPA, Porto Alegre, Brazil. 4. Infection Control Service Hospital Nossa Senhora da Conceição, Porto Alegre, Brazil. 5. Infection Control Service Hospital Beneficiência Portuguesa, Porto Alegre, Brazil. 6. Infectious Diseases Service, Hospital de Clínicas de Porto, 2350 Ramiro Barcelos St, 90035-903, Porto Alegre, Brazil; Department of Internal Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. Electronic address: azavascki@hcpa.ufrgs.br.
Abstract
OBJECTIVES: To evaluate the emergence of New Delhi metallo-β-lactamase 1 (NDM-1)-producing Enterobacteriaceae isolates in Brazil. METHODS: From April to October 2013, following the detection of the first NDM-1-producing isolate, a surveillance study was performed for the detection of blaNDM-1 among Enterobacteriaceae isolates with reduced susceptibility to carbapenems in 17 hospitals of Porto Alegre, Brazil. Real-time PCR was used to determine the presence of carbapenemase genes, which were further sequenced. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 1134 isolates were evaluated. blaNDM-1 was detected in 11 (0.97%) isolates: nine Enterobacter cloacae complex (eight belonging to a single clone recovered from two distinct hospitals and the other strain from a third hospital) and two Morganella morganii (belonging to a single clone recovered from one hospital). Most isolates presented high-level resistance to carbapenems. CONCLUSIONS: NDM-1-producing Enterobacteriaceae have emerged rapidly in the hospitals of the Brazilian city where they were first detected. The emergence of NDM-1 in Brazil is of great concern, since it is a severe threat to antimicrobial therapy against Enterobacteriaceae in this country.
OBJECTIVES: To evaluate the emergence of New Delhi metallo-β-lactamase 1 (NDM-1)-producing Enterobacteriaceae isolates in Brazil. METHODS: From April to October 2013, following the detection of the first NDM-1-producing isolate, a surveillance study was performed for the detection of blaNDM-1 among Enterobacteriaceae isolates with reduced susceptibility to carbapenems in 17 hospitals of Porto Alegre, Brazil. Real-time PCR was used to determine the presence of carbapenemase genes, which were further sequenced. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE). RESULTS: A total of 1134 isolates were evaluated. blaNDM-1 was detected in 11 (0.97%) isolates: nine Enterobacter cloacae complex (eight belonging to a single clone recovered from two distinct hospitals and the other strain from a third hospital) and two Morganella morganii (belonging to a single clone recovered from one hospital). Most isolates presented high-level resistance to carbapenems. CONCLUSIONS: NDM-1-producing Enterobacteriaceae have emerged rapidly in the hospitals of the Brazilian city where they were first detected. The emergence of NDM-1 in Brazil is of great concern, since it is a severe threat to antimicrobial therapy against Enterobacteriaceae in this country.
Authors: Melissa L Hargreaves; Kristin M Shaw; Ginette Dobbins; Paula M Snippes Vagnone; Jane E Harper; Dave Boxrud; Ruth Lynfield; Maliha Aziz; Lance B Price; Kevin A T Silverstein; Jessica L Danzeisen; Bonnie Youmans; Kyle Case; Srinand Sreevatsan; Timothy J Johnson Journal: Antimicrob Agents Chemother Date: 2015-10-05 Impact factor: 5.191