Pi Liu1, Liang Xia2, Wei-Long Zhang3, Hua-Jing Ke2, Tao Su2, Li-Bing Deng3, You-Xiang Chen2, Nong-Hua Lv4. 1. Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: liupi1974@sina.com. 2. Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. 3. Nanchang University, Nanchang 330031, China. 4. Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China. Electronic address: lunonghua@163.com.
Abstract
BACKGROUND/ OBJECTIVES: To identify serum microRNA (miRNA) as diagnostic and prognostic biomarkers for acute pancreatitis (AP). MATERIALS AND METHODS: Sera microRNA expression was profiled from 12 AP patients with varying disease severity and three healthy controls. Differentially expressed miRNAs were validated in a larger cohort of patients and controls. The diagnostic and prognostic potentials of differentially expressed miRNAs were evaluated using receiver operating characteristic (ROC) curve analysis and compared to that of classic prognostic markers for AP. RESULTS: miRNA microarray analyses identified 205 differentially expressed miRNAs between sera from AP patients and that from controls. Nine miRNAs were differentially expressed between severe and mild AP patients. Further validation confirmed the down-regulation of miR-92b, miR-10a, and miR-7 in AP patients, and ROC analysis revealed that these miRNAs can differentiate AP from health cases. Furthermore, the serum miR-551b-5p level was significantly higher in patients with disease complications or a low plasma calcium level. ROC analysis showed that the serum miR-551b-5p level can distinguish between severe and mild AP. CONCLUSION: The expressions of miR-92b, miR-10a, and miR-7 in AP might be used for the early diagnosis of AP and miR-551b-5p may be used for predicting AP severity.
BACKGROUND/ OBJECTIVES: To identify serum microRNA (miRNA) as diagnostic and prognostic biomarkers for acute pancreatitis (AP). MATERIALS AND METHODS: Sera microRNA expression was profiled from 12 AP patients with varying disease severity and three healthy controls. Differentially expressed miRNAs were validated in a larger cohort of patients and controls. The diagnostic and prognostic potentials of differentially expressed miRNAs were evaluated using receiver operating characteristic (ROC) curve analysis and compared to that of classic prognostic markers for AP. RESULTS: miRNA microarray analyses identified 205 differentially expressed miRNAs between sera from AP patients and that from controls. Nine miRNAs were differentially expressed between severe and mild AP patients. Further validation confirmed the down-regulation of miR-92b, miR-10a, and miR-7 in AP patients, and ROC analysis revealed that these miRNAs can differentiate AP from health cases. Furthermore, the serum miR-551b-5p level was significantly higher in patients with disease complications or a low plasma calcium level. ROC analysis showed that the serum miR-551b-5p level can distinguish between severe and mild AP. CONCLUSION: The expressions of miR-92b, miR-10a, and miR-7 in AP might be used for the early diagnosis of AP and miR-551b-5p may be used for predicting AP severity.
Authors: Makon-Sébastien Njock; Henry S Cheng; Lan T Dang; Maliheh Nazari-Jahantigh; Andrew C Lau; Emilie Boudreau; Mark Roufaiel; Myron I Cybulsky; Andreas Schober; Jason E Fish Journal: Blood Date: 2015-04-02 Impact factor: 22.113