Keiko Kondo1, Katsutaro Morino2, Yoshihiko Nishio3, Motoyuki Kondo1, Keiko Nakao1, Fumiyuki Nakagawa4, Atsushi Ishikado5, Osamu Sekine1, Takeshi Yoshizaki1, Atsunori Kashiwagi1, Satoshi Ugi1, Hiroshi Maegawa1. 1. Department of Medicine, Division of Endocrinology and Metabolism, Shiga University of Medical Science, Shiga, Japan. 2. Department of Medicine, Division of Endocrinology and Metabolism, Shiga University of Medical Science, Shiga, Japan. Electronic address: morino@belle.shiga-med.ac.jp. 3. Department of Diabetes and Endocrine Medicine, Division of Human and Environmental Sciences, Kagoshima University, Graduate School of Medical and Dental Sciences, Kagoshima, Japan. 4. Department of Medicine, Division of Endocrinology and Metabolism, Shiga University of Medical Science, Shiga, Japan; Osaka Laboratory, JCL Bioassay Corporation, Osaka, Japan. 5. Department of Medicine, Division of Endocrinology and Metabolism, Shiga University of Medical Science, Shiga, Japan; R&D Department, Sunstar Inc, Osaka, Japan.
Abstract
OBJECTIVE: The beneficial effects of fish and n-3 polyunsaturated fatty acids (PUFAs) consumption on atherosclerosis have been reported in numerous epidemiological studies. However, to the best of our knowledge, the effects of a fish-based diet intervention on endothelial function have not been investigated. Therefore, we studied these effects in postmenopausal women with type 2 diabetes mellitus (T2DM). MATERIALS/ METHODS:Twenty-three postmenopausal women with T2DM were assigned to two four-week periods of either a fish-based diet (n-3 PUFAs ≧ 3.0 g/day) or a control diet in a randomized crossover design. Endothelial function was measured with reactive hyperemia using strain-gauge plethysmography and compared with the serum levels of fatty acids and their metabolites. Endothelial function was determined with peak forearm blood flow (Peak), duration of reactive hyperemia (Duration) and flow debt repayment (FDR). RESULTS:A fish-based dietary intervention improved Peak by 63.7%, Duration by 27.9% and FDR by 70.7%, compared to the control diet. Serum n-3 PUFA levels increased after the fish-based diet period and decreased after the control diet, compared with the baseline (1.49 vs. 0.97 vs. 1.19 mmol/l, p < 0.0001). There was no correlation between serum n-3 PUFA levels and endothelial function. An increased ratio of epoxyeicosatrienoic acid/dihydroxyeicosatrienoic acid was observed after a fish-based diet intervention, possibly due to the inhibition of the activity of soluble epoxide hydrolase. CONCLUSIONS:A fish-based dietary intervention improves endothelial function in postmenopausal women with T2DM. Dissociation between the serum n-3 PUFA concentration and endothelial function suggests that the other factors may contribute to this phenomenon.
RCT Entities:
OBJECTIVE: The beneficial effects of fish and n-3 polyunsaturated fatty acids (PUFAs) consumption on atherosclerosis have been reported in numerous epidemiological studies. However, to the best of our knowledge, the effects of a fish-based diet intervention on endothelial function have not been investigated. Therefore, we studied these effects in postmenopausal women with type 2 diabetes mellitus (T2DM). MATERIALS/ METHODS: Twenty-three postmenopausal women with T2DM were assigned to two four-week periods of either a fish-based diet (n-3 PUFAs ≧ 3.0 g/day) or a control diet in a randomized crossover design. Endothelial function was measured with reactive hyperemia using strain-gauge plethysmography and compared with the serum levels of fatty acids and their metabolites. Endothelial function was determined with peak forearm blood flow (Peak), duration of reactive hyperemia (Duration) and flow debt repayment (FDR). RESULTS: A fish-based dietary intervention improved Peak by 63.7%, Duration by 27.9% and FDR by 70.7%, compared to the control diet. Serum n-3 PUFA levels increased after the fish-based diet period and decreased after the control diet, compared with the baseline (1.49 vs. 0.97 vs. 1.19 mmol/l, p < 0.0001). There was no correlation between serum n-3 PUFA levels and endothelial function. An increased ratio of epoxyeicosatrienoic acid/dihydroxyeicosatrienoic acid was observed after a fish-based diet intervention, possibly due to the inhibition of the activity of soluble epoxide hydrolase. CONCLUSIONS: A fish-based dietary intervention improves endothelial function in postmenopausal women with T2DM. Dissociation between the serum n-3 PUFA concentration and endothelial function suggests that the other factors may contribute to this phenomenon.
Authors: Darko Stefanovski; Pei-An Betty Shih; Bruce D Hammock; Richard M Watanabe; Jang H Youn Journal: Prostaglandins Other Lipid Mediat Date: 2020-01-10 Impact factor: 3.072
Authors: Grace Y Sun; Agnes Simonyi; Kevin L Fritsche; Dennis Y Chuang; Mark Hannink; Zezong Gu; C Michael Greenlief; Jeffrey K Yao; James C Lee; David Q Beversdorf Journal: Prostaglandins Leukot Essent Fatty Acids Date: 2017-03-10 Impact factor: 3.015