CONCLUSIONS: Dickkopf-1 (DKK1) is a novel prognostic biomarker for laryngeal squamous cell carcinoma (LSCC). DKK1 may be a promising strategy for the future treatment of LSCC metastasis and recurrence. OBJECTIVES: DKK1 is reportedly involved in the metastasis and invasion of several tumor types. This study aimed to investigate the prognostic value of DKK1 in LSCC. METHODS: DKK1 expression was measured in Hep-2 cell lines, as well as in tumor and peritumoral tissues, using quantitative real-time PCR and western blot analyses. The role of DKK1 in LSCC was investigated by depleting DKK1 using small interfering RNAs. Tissue microarrays of 102 LSCC patient samples were employed to immunohistochemically detect expression of DKK1, vascular endothelial growth factor C (VEGF-C), and β-catenin. Prognostic significance was assessed using Kaplan-Meier survival estimates. RESULTS: DKK1 expression was elevated in the Hep-2 cell line and tumor samples. DKK1 depletion decreased cell proliferation, migration, and invasiveness. High DKK1 expression was significantly associated with T and clinical stage, lymph node metastasis, and tumor size (p < 0.05). Increased DKK1 levels in LSCC tissues correlated with elevated VEGF-C and β-catenin. Multivariate analyses revealed that DKK1 was an unfavorable predictor of overall survival.
CONCLUSIONS:Dickkopf-1 (DKK1) is a novel prognostic biomarker for laryngeal squamous cell carcinoma (LSCC). DKK1 may be a promising strategy for the future treatment of LSCC metastasis and recurrence. OBJECTIVES:DKK1 is reportedly involved in the metastasis and invasion of several tumor types. This study aimed to investigate the prognostic value of DKK1 in LSCC. METHODS:DKK1 expression was measured in Hep-2 cell lines, as well as in tumor and peritumoral tissues, using quantitative real-time PCR and western blot analyses. The role of DKK1 in LSCC was investigated by depleting DKK1 using small interfering RNAs. Tissue microarrays of 102 LSCC patient samples were employed to immunohistochemically detect expression of DKK1, vascular endothelial growth factor C (VEGF-C), and β-catenin. Prognostic significance was assessed using Kaplan-Meier survival estimates. RESULTS:DKK1 expression was elevated in the Hep-2 cell line and tumor samples. DKK1 depletion decreased cell proliferation, migration, and invasiveness. High DKK1 expression was significantly associated with T and clinical stage, lymph node metastasis, and tumor size (p < 0.05). Increased DKK1 levels in LSCC tissues correlated with elevated VEGF-C and β-catenin. Multivariate analyses revealed that DKK1 was an unfavorable predictor of overall survival.
Authors: Óscar Aguilera; José Manuel González-Sancho; Sandra Zazo; Raúl Rincón; Agustín F Fernández; Olga Tapia; Francesc Canals; Beatriz Morte; Vincenzo Calvanese; José L Orgaz; Núria Niell; Susana Aguilar; José M Freije; Osvaldo Graña; David G Pisano; Aurea Borrero; Javier Martínez-Useros; Benilde Jiménez; Mario F Fraga; Jesús García-Foncillas; Carlos López-Otín; Miguel Lafarga; Federico Rojo; Alberto Muñoz Journal: Oncotarget Date: 2015-03-20
Authors: Scott M Langevin; Melissa Eliot; Rondi A Butler; Agnes Cheong; Xiang Zhang; Michael D McClean; Devin C Koestler; Karl T Kelsey Journal: Clin Epigenetics Date: 2015-12-03 Impact factor: 6.551