Adriana Pigozzo Manso1, Rosa Helena Miranda Grande2, Ana Karina Bedran-Russo3, Alessandra Reis4, Alessandro D Loguercio4, David Henry Pashley5, Ricardo Marins Carvalho6. 1. University of British Columbia, Faculty of Dentistry, Department of Oral Biological and Medical Sciences, Division of Biomaterials, Vancouver, BC, Canada; University of São Paulo, School of Dentistry, Department of Dental Biomaterials and Biochemistry, São Paulo, SP, Brazil. 2. University of São Paulo, School of Dentistry, Department of Dental Biomaterials and Biochemistry, São Paulo, SP, Brazil. 3. University of Illinois at Chicago, College of Dentistry, Department of Restorative Dentistry, Chicago, IL, USA. 4. University of Ponta Grossa, School of Dentistry, Department of Restorative Dentistry, Ponta Grossa, PR, Brazil. 5. Georgia Regents University, College of Dental Medicine, Department of Oral Biology and Maxillofacial Pathology, Augusta, GA, USA. 6. University of British Columbia, Faculty of Dentistry, Department of Oral Biological and Medical Sciences, Division of Biomaterials, Vancouver, BC, Canada. Electronic address: rickmc@dentistry.ubc.ca.
Abstract
OBJECTIVES: To examine the effects of the combined use of chlorhexidine and ethanol on the durability of resin-dentin bonds. METHODS: Forty-eight flat dentin surfaces were etched (32% phosphoric acid), rinsed (15 s) and kept wet until bonding procedures. Dentin surfaces were blot-dried with absorbent paper and re-wetted with water (water, control), 1% chlorhexidine diacetate in water (CHD/water), 100% ethanol (ethanol), or 1% chlorhexidine diacetate in ethanol (CHD/ethanol) solutions for 30 s. They were then bonded with All Bond 3 (AB3, Bisco) or Excite (EX, Ivoclar-Vivadent) using a smooth, continuous rubbing application (10 s), followed by 15 s gentle air stream to evaporate solvents. The adhesives were light-cured (20 s) and resin composite build-ups constructed for the microtensile method. Bonded beams were obtained and tested after 24-h, 6-months and 15-months of water storage at 37°C. Storage water was changed every month. Effects of treatment and testing periods were analyzed (ANOVA, Holm-Sidak, p<0.05) for each adhesive. RESULTS: There were no interactions between factors for both etch-and-rinse adhesives. AB3 was significantly affected only by storage (p=0.003). Excite was significantly affected only by treatments (p=0.048). AB3 treated either with ethanol or CHD/ethanol resulted in reduced bond strengths after 15 months. The use of CHD/ethanol resulted in higher bond strengths values for Excite. CONCLUSIONS: Combined use of ethanol/1% chlorhexidine diacetate did not stabilize bond strengths after 15 months.
OBJECTIVES: To examine the effects of the combined use of chlorhexidine and ethanol on the durability of resin-dentin bonds. METHODS: Forty-eight flat dentin surfaces were etched (32% phosphoric acid), rinsed (15 s) and kept wet until bonding procedures. Dentin surfaces were blot-dried with absorbent paper and re-wetted with water (water, control), 1% chlorhexidine diacetate in water (CHD/water), 100% ethanol (ethanol), or 1% chlorhexidine diacetate in ethanol (CHD/ethanol) solutions for 30 s. They were then bonded with All Bond 3 (AB3, Bisco) or Excite (EX, Ivoclar-Vivadent) using a smooth, continuous rubbing application (10 s), followed by 15 s gentle air stream to evaporate solvents. The adhesives were light-cured (20 s) and resin composite build-ups constructed for the microtensile method. Bonded beams were obtained and tested after 24-h, 6-months and 15-months of water storage at 37°C. Storage water was changed every month. Effects of treatment and testing periods were analyzed (ANOVA, Holm-Sidak, p<0.05) for each adhesive. RESULTS: There were no interactions between factors for both etch-and-rinse adhesives. AB3 was significantly affected only by storage (p=0.003). Excite was significantly affected only by treatments (p=0.048). AB3 treated either with ethanol or CHD/ethanol resulted in reduced bond strengths after 15 months. The use of CHD/ethanol resulted in higher bond strengths values for Excite. CONCLUSIONS: Combined use of ethanol/1% chlorhexidine diacetate did not stabilize bond strengths after 15 months.
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