Unraveling architecture of the pluripotent genome.

Genomes are folded into sophisticated configurations that both shape, and are shaped by, a diverse range of nuclear functions. High-throughput variations of Chromosome-Conformation-Capture-based technologies now enable analysis of architecture at unprecedented resolution and scale. Here I discuss the complex structure-function relationship of the mammalian genome using the model system of embryonic stem cells, and the progression from pluripotency to terminal differentiation and back again.