Agnieszka Jabłońska1, Edyta Paradowska2, Mirosława Studzińska1, Patrycja Suski1, Dorota Nowakowska3, Małgorzata Wiśniewska-Ligier4, Teresa Woźniakowska-Gęsicka4, Jan Wilczyński3, Zbigniew J Leśnikowski1. 1. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland. 2. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, 106 Lodowa St., 93-232 Lodz, Poland. Electronic address: eparadowska@cbm.pan.pl. 3. Department of Foetal-Maternal Medicine and Gynaecology, Third Chair of Gynaecology and Obstetrics, Medical University, Lodz, Poland; Department of Foetal-Maternal Medicine and Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland. 4. Third Department of Paediatrics, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Abstract
OBJECTIVES: The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults. METHODS: The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR. RESULTS: Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p<0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found. CONCLUSION: Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
OBJECTIVES: The association among specific single-nucleotide polymorphisms (SNPs) in TLR2 (Arg677Trp, Arg753Gln) and TLR4 (Asp299Gln) and human cytomegalovirus (CMV) infection was studied in infants and adults. METHODS: The TLR2 and TLR4 polymorphisms were genotyped in 151 patients with CMV infections and in 78 unrelated healthy individuals. Genotyping was performed by restriction fragment length polymorphism (RFLP) analysis of PCR-amplified fragments. The viral load was measured by quantitative real-time PCR. RESULTS: Almost all of the patients with CMV infections were wild-type homozygotes without TLR2 and TLR4 polymorphisms. No significant differences in TLR2 and TLR4 polymorphisms were observed between infants with or without CMV infection. Compared with adults with CMV infections, heterozygosity for the TLR2 Arg677Trp and TLR4 Asp299Gly SNPs was detected more frequently in healthy individuals (p<0.05). Logistic regression analysis showed that the wild-type TLR2 genotype was associated with an increased risk of CMV infection and that heterozygosity for TLR2 and TLR4 SNPs diminished the risk of CMV infection in adult patients. An association between CMV load and the TLR4 SNP was found. CONCLUSION: Our results suggest that the wild-type TLR2 genotype may be a risk factor for CMV replication in adult patients.
Authors: Martina Schneider; Teresa Matiqi; Michael Kundi; Franz J J Rieder; Martin Andreas; Robert Strassl; Andreas Zuckermann; Christof Jungbauer; Christoph Steininger Journal: J Clin Virol Date: 2016-10-04 Impact factor: 3.168