| Literature DB >> 2480180 |
Abstract
The properties of an optical microscope are analyzed and analytically evaluated with a simple and effective model in order to understand the true meaning, limitations, and real capabilities of a defocusing technique. Major emphasis is given to the applications related to microscopic objects of biological interest using fluorescence and absorption light microscopy. A procedure for three-dimensional viewing is analyzed and discussed.Mesh:
Year: 1989 PMID: 2480180 DOI: 10.1007/bf02989683
Source DB: PubMed Journal: Cell Biophys ISSN: 0163-4992