Effects of chicken-liver hydrolysates on lipid metabolism in a high-fat diet.

The contents of free hydrophobic amino acids, taurine and carnosine/anserine were elevated after hydrolyzing chicken livers by pepsin and compared to dried chicken livers. Chicken-liver-hydrolysates (CLHs) exhibited in vitro inhibitory lipase activity and bile-acid binding ability (p<0.05). Forty-eight male hamsters were assigned randomly to the following groups: (1) chow diet; (2) high-fat diet (HFD); (3) HFD and 100 mg CLH/kg BW; (4) HFD and 200 mg CLH/kg BW; (5) HFD and 400 mg CLH/kg BW; (6) HFD and 200 mg carnosine/kg BW. CLHs alleviated (p<0.05) serum oxidative stress and improved (p<0.05) the serum lipid profile in the high-fat dietary groups; meanwhile, improved (p<0.05) antioxidant abilities and decreased (p<0.05) lipid accumulation, oxidative stress and TNF-α/IL-1β levels in the livers. These benefits might result from regulations of lipid homeostasis and increased faecal bile-acid outputs (p<0.05). Hence, lipid-homeostasis and antioxidant abilities of CLHs in the high-fat dietary habit were demonstrated and were similar to pure carnosine.