| Literature DB >> 24796604 |
Sander B Engelsma1, Lianne I Willems, Claudia E van Paaschen, Sander I van Kasteren, Gijsbert A van der Marel, Herman S Overkleeft, Dmitri V Filippov.
Abstract
A new bioorthogonal N-acylazetine tag, suitable for tetrazine mediated inverse electron-demand Diels-Alder conjugation, is developed. The tag is small and achiral. We demonstrate the usefulness of N-acylazetine-tetrazine based bioorthogonal chemistry in two-step activity-based protein profiling. The performance of the new tetrazinophile in the labeling of catalytically active proteasome subunits was comparable to that of the more sterically demanding norbornene tag.Entities:
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Year: 2014 PMID： 24796604 DOI： 10.1021/ol501049c
Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005