Literature DB >> 24789178

Comparative evaluation of the Diagenode multiplex PCR assay on the BD max system versus a routine in-house assay for detection of Bordetella pertussis.

Juliet Kenicer1, Alison Hardie1, Fiona Hamilton1, Naomi Gadsby1, Kate Templeton2.   

Abstract

This study looked at 128 nasopharyngeal aspirates (NPA) and 162 throat swabs (TS) tested with the Diagenode multiplex assay on the BD Max system versus our in-house Bordetella pertussis PCR. Sensitivity and specificity were 97.3% and 100% for NPA and 88.3% and 98% for TS, respectively. Of positive NPA, 42.1% were coinfected with respiratory viruses.
Copyright © 2014, American Society for Microbiology. All Rights Reserved.

Mesh：

Year:  2014        PMID： 24789178      PMCID： PMC4097751          DOI： 10.1128/JCM.00212-14

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.

Authors:  Julian Parkhill; Mohammed Sebaihia; Andrew Preston; Lee D Murphy; Nicholas Thomson; David E Harris; Matthew T G Holden; Carol M Churcher; Stephen D Bentley; Karen L Mungall; Ana M Cerdeño-Tárraga; Louise Temple; Keith James; Barbara Harris; Michael A Quail; Mark Achtman; Rebecca Atkin; Steven Baker; David Basham; Nathalie Bason; Inna Cherevach; Tracey Chillingworth; Matthew Collins; Anne Cronin; Paul Davis; Jonathan Doggett; Theresa Feltwell; Arlette Goble; Nancy Hamlin; Heidi Hauser; Simon Holroyd; Kay Jagels; Sampsa Leather; Sharon Moule; Halina Norberczak; Susan O'Neil; Doug Ormond; Claire Price; Ester Rabbinowitsch; Simon Rutter; Mandy Sanders; David Saunders; Katherine Seeger; Sarah Sharp; Mark Simmonds; Jason Skelton; Robert Squares; Steven Squares; Kim Stevens; Louise Unwin; Sally Whitehead; Bart G Barrell; Duncan J Maskell
Journal:  Nat Genet       Date:  2003-08-10       Impact factor: 38.330

2.  External quality assessment for molecular detection of Bordetella pertussis in European laboratories.

Authors:  G Muyldermans; O Soetens; M Antoine; S Bruisten; B Vincart; F Doucet-Populaire; N K Fry; P Olcén; J M Scheftel; J M Senterre; A van der Zee; M Riffelmann; D Piérard; S Lauwers
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

3.  Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.

Authors:  Karen B Register; Gary N Sanden
Journal:  J Clin Microbiol       Date:  2006-10-25       Impact factor: 5.948

4.  Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii.

Authors:  U Reischl; N Lehn; G N Sanden; M J Loeffelholz
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

5.  Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction.

Authors:  E M Glare; J C Paton; R R Premier; A J Lawrence; I T Nisbet
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

6.  Polymerase chain reaction assay for pertussis: simultaneous detection and discrimination of Bordetella pertussis and Bordetella parapertussis.

Authors:  A van der Zee; C Agterberg; M Peeters; J Schellekens; F R Mooi
Journal:  J Clin Microbiol       Date:  1993-08       Impact factor: 5.948

7.  Evaluation of PCR methods for the diagnosis of pertussis by the European surveillance network for vaccine-preventable diseases (EUVAC.NET).

Authors:  T Dalby; N K Fry; K A Krogfelt; J S Jensen; Q He
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2013-04-23       Impact factor: 3.267

8.  Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis.

Authors:  Kate E Templeton; Sitha A Scheltinga; Anneke van der Zee; Bram M W Diederen; Alida M van Kruijssen; Herman Goossens; Ed Kuijper; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

9.  Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae.

Authors:  Kate E Templeton; Sitha A Scheltinga; A Willy Graffelman; Jolanda M Van Schie; Jantine W Crielaard; Peter Sillekens; Peterhans J Van Den Broek; Herman Goossens; Matthias F C Beersma; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

10.  Improved diagnosis of the etiology of community-acquired pneumonia with real-time polymerase chain reaction.

Authors:  Kate E Templeton; Sitha A Scheltinga; Willian C J F M van den Eeden; A Willy Graffelman; Peterhans J van den Broek; Eric C J Claas
Journal:  Clin Infect Dis       Date:  2005-06-22       Impact factor: 9.079
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  2 in total

1.  Development and Validation of a Laboratory-Developed Multiplex Real-Time PCR Assay on the BD Max System for Detection of Herpes Simplex Virus and Varicella-Zoster Virus DNA in Various Clinical Specimens.

Authors:  Sylvie Pillet; Paul O Verhoeven; Amélie Epercieux; Thomas Bourlet; Bruno Pozzetto
Journal:  J Clin Microbiol       Date:  2015-04-15       Impact factor: 5.948

Review 2.  Laboratory Diagnosis of Pertussis.

Authors:  Anneke van der Zee; Joop F P Schellekens; Frits R Mooi
Journal:  Clin Microbiol Rev       Date:  2015-10       Impact factor: 26.132
  2 in total

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